Virus-like particles induce robust human T-helper cell responses

Braun, Marion; Jandus, Camilla; Maurer, Patrik; Hammann-Haenni, Anya; Schwarz, Katrin; Bachmann, Martin F; Speiser, Daniel E; Romero, Pedro (2012). Virus-like particles induce robust human T-helper cell responses. European journal of immunology, 42(2), pp. 330-40. Weinheim: Wiley-VCH 10.1002/eji.201142064

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Among synthetic vaccines, virus-like particles (VLPs) are used for their ability to induce strong humoral responses. Very little is reported on VLP-based-vaccine-induced CD4(+) T-cell responses, despite the requirement of helper T cells for antibody isotype switching. Further knowledge on helper T cells is also needed for optimization of CD8(+) T-cell vaccination. Here, we analysed human CD4(+) T-cell responses to vaccination with MelQbG10, which is a Qβ-VLP covalently linked to a long peptide derived from the melanoma self-antigen Melan-A. In all analysed patients, we found strong antibody responses of mainly IgG1 and IgG3 isotypes, and concomitant Th1-biased CD4(+) T-cell responses specific for Qβ. Although less strong, comparable B- and CD4(+) T-cell responses were also found specific for the Melan-A cargo peptide. Further optimization is required to shift the response more towards the cargo peptide. Nevertheless, the data demonstrate the high potential of VLPs for inducing humoral and cellular immune responses by mounting powerful CD4(+) T-cell help.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Pharmacology

UniBE Contributor:

Jandus, Camilla

ISSN:

0014-2980

Publisher:

Wiley-VCH

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:18

Last Modified:

17 Mar 2015 19:23

Publisher DOI:

10.1002/eji.201142064

PubMed ID:

22057679

Web of Science ID:

000299337800009

URI:

https://boris.unibe.ch/id/eprint/5469 (FactScience: 210216)

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