Mitochondrial outer membrane proteome of T.brucei reveals novel factors required to maintain mitochondrial morphology

Niemann, Moritz (5 May 2013). Mitochondrial outer membrane proteome of T.brucei reveals novel factors required to maintain mitochondrial morphology (Unpublished). In: Cell Symposia - Mitochondria: from Signaling to Disease. Lissabon, Portugal. 05.-07.05.2013.

African trypanosomes, the causative agent of Human African Trypanosomiasis (HAT) are among the earliest diverging eukaryotes that have bona fide mitochondria capable of oxidative phosphorylation. The mitochondrial outer membrane (MOM) of T. brucei is essentially unchartered territory. The beta barrel membrane proteins VDAC, Sam50 and archaic TOM are the only MOM proteins that have been characterized so far. Using biochemical fractionation and correlated protein abundance-profiling we were able to raise the protein inventory of the MOM. Of the 82 candidate proteins two-thirds have never been associated with mitochondria before. The function of 42 proteins remains unknown. Known factors involved in the regulation of mitochondrial morphology are virtually absent in T. brucei. Interestingly, RNAi-mediated ablation of three MOM candidate proteins of unknown function resulted in a collapse of the network-like mitochondrion of insect-stage parasites and therefore directly or indirectly are involved in the regulation of mitochondrial morphology in T. brucei.

Item Type:

Conference or Workshop Item (Poster)

Division/Institute:

08 Faculty of Science > Departement of Chemistry and Biochemistry

UniBE Contributor:

Niemann, Moritz

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry

Language:

English

Submitter:

Christina Schüpbach

Date Deposited:

05 Aug 2014 13:32

Last Modified:

05 Aug 2014 13:32

URI:

https://boris.unibe.ch/id/eprint/57495

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