Impaired genome encapsidation restricts the in vitro propagation of human parvovirus B19.

Wolfisberg, Raphael; Ruprecht, Nico; Kempf, Christoph; Ros, Carlos (2013). Impaired genome encapsidation restricts the in vitro propagation of human parvovirus B19. Journal of virological methods, 193(1), pp. 215-225. Elsevier 10.1016/j.jviromet.2013.06.003

[img] Text
1-s2.0-S0166093413002164-main_Wolfisberg_Ruprecht_Kempf_Ros.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (3MB) | Request a copy

The lack of a permissive cell culture system hampers the study of human parvovirus B19 (B19V). UT7/Epo is one of the few established cell lines that can be infected with B19V but generates none or few infectious progeny. Recently, hypoxic conditions or the use of primary CD36+ erythroid progenitor cells (CD36+ EPCs) have been shown to improve the infection. These novel approaches were evaluated in infection and transfection experiments. Hypoxic conditions or the use of CD36+ EPCs resulted in a significant acceleration of the infection/transfection and a modest increase in the yield of capsid progeny. However, under all tested conditions, genome encapsidation was impaired seriously. Further analysis of the cell culture virus progeny revealed that differently to the wild-type virus, the VP1 unique region (VP1u) was exposed partially and was unable to become further externalized upon heat treatment. The fivefold axes pore, which is used for VP1u externalization and genome encapsidation, might be constricted by the atypical VP1u conformation explaining the packaging failure. Although CD36+ EPCs and hypoxia facilitate B19V infection, large quantities of infectious progeny cannot be generated due to a failure in genome encapsidation, which arises as a major limiting factor for the in vitro propagation of B19V.

Item Type:

Journal Article (Original Article)


08 Faculty of Science > Department of Chemistry, Biochemistry and Pharmaceutical Sciences (DCBP)

UniBE Contributor:

Wolfisberg, Raphael; Ruprecht, Nico; Kempf, Christoph and Ros Bascunana, Carlos


500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry








Christoph Kempf

Date Deposited:

01 Sep 2014 13:45

Last Modified:

31 May 2021 11:56

Publisher DOI:


PubMed ID:


Uncontrolled Keywords:

EPCs, Erythroid progenitor cells, Hypoxia, Parvovirus B19, UT7/Epo cells, VP1u




Actions (login required)

Edit item Edit item
Provide Feedback