Humoral immune response to ADAMTS13 in acquired thrombotic thrombocytopenic purpura

Pos, W; Luken, B M; Sorvillo, N; Hovinga, J A Kremer; Voorberg, J (2011). Humoral immune response to ADAMTS13 in acquired thrombotic thrombocytopenic purpura. Journal of thrombosis and haemostasis, 9(7), pp. 1285-91. Oxford: Wiley-Blackwell 10.1111/j.1538-7836.2011.04307.x

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The apparently spontaneous development of autoantibodies to ADAMTS13 in previously healthy individuals is a major cause of thrombotic thrombocytopenic purpura (TTP). Epitope mapping studies have shown that in most patients antibodies directed towards the spacer domain of ADAMTS13 are present. A single antigenic surface comprising Arg(660) , Tyr(661) and Tyr(665) that contributes to the productive binding of ADAMTS13 to unfolded von Willebrand factor is targeted by anti-spacer domain antibodies. Antibodies directed to the carboxyl-terminal CUB1-2 and TSP2-8 domains have also been observed in the plasma of patients with acquired TTP. As yet it has not been established whether this class of antibodies modulates ADAMTS13 activity. Inspection of the primary sequence of human monoclonal anti-ADAMTS13 antibodies suggests that the variable heavy chain germline gene segment VH1-69 is frequently incorporated. We suggest a model in which 'shape complementarity' between the spacer domain and residues encoded by the VH1-69 gene segment explain the preferential use of this variable heavy chain gene segment. Finally, a model is presented for the development of anti-ADAMTS13 antibodies in previously healthy individuals that incorporates the recent identification of HLA DRB1*11 as a risk factor for acquired TTP.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Clinic of Haematology and Central Haematological Laboratory

UniBE Contributor:

Kremer Hovinga, Johanna Anna

ISSN:

1538-7836

Publisher:

Wiley-Blackwell

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:18

Last Modified:

16 Jul 2018 14:14

Publisher DOI:

10.1111/j.1538-7836.2011.04307.x

PubMed ID:

21535387

Web of Science ID:

000292336600002

URI:

https://boris.unibe.ch/id/eprint/5847 (FactScience: 210700)

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