In vitro and in vivo activity of human interleukin-8 in dogs

Zwahlen, R D; Spreng, David; Wyder-Walther, M (1994). In vitro and in vivo activity of human interleukin-8 in dogs. Veterinary pathology, 31(1), pp. 61-66. 10.1177/030098589403100108

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Interleukin-8 (IL-8), a proinflammatory cytokine produced by human monocytes, fibroblasts, and endothelial and epithelial cells, is effective not only on cells and tissues of human beings but also on those of several animal species. We investigated the importance of recombinant human IL-8 for the activation of canine neutrophils in vitro and its potential for inducing inflammation in vivo. Shape change (10(-9)-10(-7) M IL-8) and chemotaxis (10(-10)-10(-6) M IL-8) assays were used to determine the activation of canine neutrophils in vitro. Chemotaxis was induced by IL-8 at doses > 10(-8) M with a maximum response at 10(-6) M. A rapid shape change of comparable intensity was elicited by 10(-9)-10(-7) M IL-8. Thirty minutes after intradermal injection of 10(-9) moles of IL-8, emigration of neutrophils could be observed and became more intense at 60 minutes and 240 minutes, respectively. Zymosan-activated canine plasma, which served as a positive control, induced a rapid, massive, and more diffuse neutrophil accumulation, whereas the reaction after IL-8 was weaker but still significant. The neutrophil accumulation after IL-8 was preferentially located in perivenular areas of the deep dermis. Recombinant human IL-8 is capable of activating canine neutrophils in vitro and is able to generate significant neutrophil accumulation in dog skin. Its activity is lower than that in human, rabbit, and rat systems.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Small Animal Clinic > Small Animal Clinic, Surgery
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Small Animal Clinic

UniBE Contributor:

Spreng, David

Subjects:

500 Science

ISSN:

0300-9858

Language:

English

Submitter:

Simone Forterre

Date Deposited:

11 Sep 2014 12:01

Last Modified:

11 Sep 2014 12:01

Publisher DOI:

10.1177/030098589403100108

PubMed ID:

8140727

URI:

https://boris.unibe.ch/id/eprint/58554

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