The effects of doxycycline on nitric oxide and stromelysin production in dogs with cranial cruciate ligament rupture

Jauernig, S.; Schweighauser, Ariane; Reist, M.; Von Rechenberg, Brigitte; Schawalder, Peter; Spreng, David (1999). The effects of doxycycline on nitric oxide and stromelysin production in dogs with cranial cruciate ligament rupture. Veterinary surgery, 30(2), pp. 132-139. Wiley-Blackwell

Text jvet.2001.21399.pdf - Published Version Restricted to registered users only Available under License Publisher holds Copyright. Download (43kB)

OBJECTIVE

To investigate the potential of doxycycline to reduce stromelysin and inducible nitric oxide synthase (iNOS) activity in dogs with osteoarthritis (OA) secondary to spontaneous cranial cruciate ligament (CCL) rupture.

STUDY DESIGN

Prospective, clinical study.

ANIMALS

Eighty-one dogs with OA secondary to CCL rupture and 54 normal dogs.

METHODS

Dogs with OA secondary to CCL rupture were divided into 2 groups before surgery. The Doxy-CCl group received 3 to 4 mg/kg doxycycline orally every 24 hours for 7 to 10 days (n = 35). The CCL group received no treatment (n = 46). Synovial fluid, articular cartilage, synovial membrane, and CCL samples were collected during surgery (Doxy-CCL group and CCL group) or immediately after euthanasia from healthy dogs (control group). Synovial fluid samples were examined cytologically. Total nitric oxide (NOt) concentrations were measured in the supernatant of explant cultures of all tissue samples, and stromelysin activity was measured in the supernatant of explant cultures of cartilage.

RESULTS

NOt concentrations measured in cartilage were significantly lower in the Doxy-CCL group than in the CCL group, but were not different from those measured in the control group. Doxycycline treatment did not have a significant effect on cartilage stromelysin levels.

CONCLUSION

The findings in this study indicate that doxycycline inhibits NO production in cartilage in dogs with CCL rupture.

CLINICAL RELEVANCE

Doxycycline may have a role in the treatment of canine OA by inhibiting NO production.

Interest & Impact

Downloads

1 since deposited on 15 Sep 2014
0 in the past 12 months

Citations

5 Citations in Web of Science ®
6 Citations in Scopus

Search

in Google Scholar™

Services

Actions (login required)

Edit item

Actions (login required)

Edit item