Cultivation and expansion of canine Schwann cells using reexplantation

Pauls, Johanna; Nolte, Christiane; Forterre, Franck; Brunnberg, Leo (2004). Cultivation and expansion of canine Schwann cells using reexplantation. Berliner und Münchener tierärztliche Wochenschrift, 117(7-8), pp. 341-352. Schlütersche

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Despite the numerous available possibilities for the surgical treatment of peripheral nerve lesions found in the dog, the success of these treatments is often unsatisfactory. It has been proven that Schwann cells (SC) have a positive influence on the regeneration of nerve stumps. Implanting a guidance channel seeded with autologous SC at the lesion site could be a new therapeutic approach. The aim of this research was to investigate the in vitro cultivation and expansion of canine SC as the main requirement for the treatment referred to above. Biopsies were carried out on 17 nerve samples originating from dogs of different breed, age, gender and condition. The reexplantation method was employed, followed by dissociation using hyaluronidase, collagenase and trypsin and further expansion. The samples were divided into six groups which were treated with a varying combination of mitogens (forskolin, bovine PEX, choleratoxin, heregulin). To obtain the quantities of SC, the specimens were immunostained by a p75-antibody. By employing a growing number of agents it was possible to obtain an increase in both the quantity of cells and purity of cultures. A maximum of 16x10(5) cells per millilitre of suspension was achieved. The largest SC purity measured 27.1%. The maximum SC quantity achieved was 43.3x10(4) SC per millilitre.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Small Animal Clinic > Small Animal Clinic, Surgery
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Small Animal Clinic

UniBE Contributor:

Forterre, Franck

Subjects:

500 Science
600 Technology > 630 Agriculture

ISSN:

0005-9366

Publisher:

Schlütersche

Language:

German

Submitter:

Simone Forterre

Date Deposited:

26 Sep 2014 09:17

Last Modified:

26 Sep 2014 09:17

PubMed ID:

15298063

URI:

https://boris.unibe.ch/id/eprint/58778

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