Influence of hormone replacement therapy on proteomic pattern in serum of postmenopausal women

Gericke, Beate; Koebnick, Corinna; Reimann, Manja; Forterre, Simone; Franz Zunft, H.J.; Schweigert, Florian J. (2005). Influence of hormone replacement therapy on proteomic pattern in serum of postmenopausal women. Maturitas, 51(4), pp. 334-342. Elsevier 10.1016/j.maturitas.2004.08.016

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OBJECTIVES: Proteomics approaches to cardiovascular biology and disease hold the promise of identifying specific proteins and peptides or modification thereof to assist in the identification of novel biomarkers. METHOD: By using surface-enhanced laser desorption and ionization time of flight mass spectroscopy (SELDI-TOF-MS) serum peptide and protein patterns were detected enabling to discriminate between postmenopausal women with and without hormone replacement therapy (HRT). RESULTS: Serum of 13 HRT and 27 control subjects was analyzed and 42 peptides and proteins could be tentatively identified based on their molecular weight and binding characteristics on the chip surface. By using decision tree-based Biomarker Patternstrade mark Software classification and regression analysis a discriminatory function was developed allowing to distinguish between HRT women and controls correctly and, thus, yielding a sensitivity of 100% and a specificity of 100%. The results show that peptide and protein patterns have the potential to deliver novel biomarkers as well as pinpointing targets for improved treatment. The biomarkers obtained represent a promising tool to discriminate between HRT users and non-users. CONCLUSION: According to a tentative identification of the markers by their molecular weight and binding characteristics, most of them appear to be part of the inflammation induced acute-phase response

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV)
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Small Animal Clinic > Small Animal Clinic, Surgery
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Small Animal Clinic

UniBE Contributor:

Forterre, Simone

Subjects:

600 Technology > 610 Medicine & health
600 Technology > 630 Agriculture

ISSN:

0378-5122

Publisher:

Elsevier

Language:

English

Submitter:

Simone Forterre

Date Deposited:

24 Sep 2014 15:21

Last Modified:

25 Dec 2014 01:11

Publisher DOI:

10.1016/j.maturitas.2004.08.016

PubMed ID:

16039405

BORIS DOI:

10.7892/boris.58812

URI:

https://boris.unibe.ch/id/eprint/58812

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