Protein profiling of urine from dogs with renal disease using ProteinChip analysis

Forterre, Simone; Raila, Jens; Schweigert, Florian J. (2004). Protein profiling of urine from dogs with renal disease using ProteinChip analysis. Journal of veterinary diagnostic investigation, 16(4), pp. 271-277. American Association of Veterinary Laboratory Diagnosticians AAVLD

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Measurement of total urinary proteins in individuals that tested positive by urinary dipstick is a typical method for assessing the presence of potentially serious renal disorders. In the absence of such overt proteinuria, however, measurement of specific urinary proteins may be useful in the diagnosis of nephropathies and may provide greater insight into the pathogenesis. The urine of 28 dogs (16 with renal disease and 12 healthy) was evaluated to determine whether specific low-molecular-weight proteins or the pattern of protein excretion could also be used as a marker of tubular dysfunction in dogs. Specific proteins were assessed by immunological methods, whereas protein profiles were determined by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (MS). In particular, changes in the excretion of retinol-binding protein (RBP) and Tamm-Horsfall protein (THP) appear to be of clinical relevance in the diagnosis of canine kidney diseases. The pattern of urinary protein and peptides revealed specific changes in abundance in dogs with renal disease at molecular masses (kD) of 11.58, 12.41, 12.60, 14.58, 20.95 (RBP), 27.85, and 65.69 (albumin). In conclusion, comparable proteins as in humans might be used as urinary markers for proximal (RBP) and distal (THP) tubular dysfunction in dogs. Surface-enhanced laser desorption/ionization time-of-flight MS is a promising tool for the study of kidney physiology and pathophysiology and might aid in the discovery of new biomarkers of renal disease.

Item Type:	Journal Article (Original Article)
Division/Institute:	05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Small Animal Clinic > Small Animal Clinic, Surgery 05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Small Animal Clinic
UniBE Contributor:	Forterre, Simone
Subjects:	600 Technology > 610 Medicine & health 600 Technology > 630 Agriculture
ISSN:	1040-6387
Publisher:	American Association of Veterinary Laboratory Diagnosticians AAVLD
Language:	English
Submitter:	Simone Forterre
Date Deposited:	24 Sep 2014 15:24
Last Modified:	05 Dec 2022 14:37
PubMed ID:	15305736
URI:	https://boris.unibe.ch/id/eprint/58814