The potential of microfluidic lung epithelial wounding: towards in vivo-like alveolar microinjuries

Felder, Marcel; Stucki, Andreas; Stucki, Janick; Geiser, Thomas; Guenat, Olivier Thierry (2014). The potential of microfluidic lung epithelial wounding: towards in vivo-like alveolar microinjuries. Integrative biology : quantitative biosciences from nano to macro, 6(12), pp. 1132-1140. Royal Society of Chemistry 10.1039/c4ib00149d

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Idiopathic pulmonary fibrosis (IPF) remains a major clinical challenge to date. Repeated alveolar epithelial microinjuries are considered as the starting point and the key event in both the development and the progression of IPF. Various pro-fibrotic agents have been identified and shown to cause alveolar damage. In IPF, however, no leading cause of alveolar epithelial microinjuries can be identified and the exact etiology remains elusive. New results from epidemiologic studies suggest a causal relation between IPF and frequent episodes of gastric refluxes resulting in gastric microaspirations into the lung. The effect of gastric contents on the alveolar epithelium has not been investigated in detail. Here, we present a microfluidic lung epithelial wounding system that allows for the selective exposure of alveolar epithelial cells to gastric contents. The system is revealed to be robust and highly reproducible. The thereby created epithelial microwounds are of tiny dimensions and best possibly reproduce alveolar damage in the lung. We further demonstrate that exposure to gastric contents, namely hydrochloric acid (HCl) and pepsin, directly damages the alveolar epithelium. Together, this novel in vitro wounding system allows for the creation of in vivo-like alveolar microinjuries with the potential to study lung injury and alveolar wound repair in vitro.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Thoracic Surgery
04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Pneumology
10 Strategic Research Centers > ARTORG Center for Biomedical Engineering Research > ARTORG Center - Lung Regeneration Technologies
09 Interdisciplinary Units > Microscopy Imaging Center MIC

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Felder, Marcel; Stucki, Andreas; Stucki, Janick; Geiser, Thomas and Guenat, Olivier Thierry

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1757-9708

Publisher:

Royal Society of Chemistry

Language:

English

Submitter:

Olivier Thierry Guenat

Date Deposited:

01 Oct 2014 10:56

Last Modified:

15 Feb 2019 09:07

Publisher DOI:

10.1039/c4ib00149d

PubMed ID:

25205504

BORIS DOI:

10.7892/boris.58851

URI:

https://boris.unibe.ch/id/eprint/58851

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