An in vitro toxicity evaluation of gold-, PLLA- and PCL-coated silica nanoparticles in neuronal cells for nanoparticle-assisted laser-tissue soldering.

Koch, Franziska; Möller, Anja-Maria; Frenz, Martin; Pieles, Uwe; Kühni, Kathrin; Mevissen, Meike (2014). An in vitro toxicity evaluation of gold-, PLLA- and PCL-coated silica nanoparticles in neuronal cells for nanoparticle-assisted laser-tissue soldering. Toxicology in vitro, 28(5), pp. 990-998. Elsevier 10.1016/j.tiv.2014.04.010

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The uptake of silica (Si) and gold (Au) nanoparticles (NPs) engineered for laser-tissue soldering in the brain was investigated using microglial cells and undifferentiated and differentiated SH-SY5Y cells. It is not known what effects NPs elicit once entering the brain. Cellular uptake, cytotoxicity, apoptosis, and the potential induction of oxidative stress by means of depletion of glutathione levels were determined after NP exposure at concentrations of 10(3) and 10(9)NPs/ml. Au-, silica poly (ε-caprolactone) (Si-PCL-) and silica poly-L-lactide (Si-PLLA)-NPs were taken up by all cells investigated. Aggregates and single NPs were found in membrane-surrounded vacuoles and the cytoplasm, but not in the nucleus. Both NP concentrations investigated did not result in cytotoxicity or apoptosis, but reduced glutathione (GSH) levels predominantly at 6 and 24h, but not after 12 h of NP exposure in the microglial cells. NP exposure-induced GSH depletion was concentration-dependent in both cell lines. Si-PCL-NPs induced the strongest effect of GSH depletion followed by Si-PLLA-NPs and Au-NPs. NP size seems to be an important characteristic for this effect. Overall, Au-NPs are most promising for laser-assisted vascular soldering in the brain. Further studies are necessary to further evaluate possible effects of these NPs in neuronal cells.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > NeuroCenter
08 Faculty of Science > Institute of Applied Physics
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Pharmacology and Toxicology
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Anatomy
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH)
09 Interdisciplinary Units > Microscopy Imaging Center MIC

UniBE Contributor:

Koch, Franziska; Möller, Anja-Maria; Frenz, Martin; Kühni, Kathrin and Mevissen, Meike

Subjects:

600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology
600 Technology > 620 Engineering

ISSN:

0887-2333

Publisher:

Elsevier

Language:

English

Submitter:

Michael Hubert Stoffel

Date Deposited:

15 Oct 2014 15:41

Last Modified:

22 Jan 2019 13:21

Publisher DOI:

10.1016/j.tiv.2014.04.010

PubMed ID:

24768613

Uncontrolled Keywords:

Gold, Nanoparticle, Neuronal cells, Neurotoxicity, Oxidative stress, Silica

BORIS DOI:

10.7892/boris.58963

URI:

https://boris.unibe.ch/id/eprint/58963

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