Genotypes and antibiotic resistances of Campylobacter jejuni isolates from cattle and pigeons in dairy farms.

Bianchini, Valentina; Luini, Mario; Borella, Laura; Parisi, Antonio; Jonas, Romie; Kittl, Sonja; Kuhnert, Peter (2014). Genotypes and antibiotic resistances of Campylobacter jejuni isolates from cattle and pigeons in dairy farms. International journal of environmental research and public health, 11(7), pp. 7154-7162. Molecular Diversity Preservation International MDPI 10.3390/ijerph110707154

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Campylobacter jejuni is the most common food-borne zoonotic pathogen causing human gastroenteritis worldwide and has assumed more importance in Italy following the increased consumption of raw milk. Our objectives were to get an overview of genotypes and antibiotic resistances in C. jejuni isolated from milk, cattle feces, and pigeons in dairy herds of Northern Italy. flaB-typing was applied to 78 C. jejuni isolates, previously characterized by Multi-Locus Sequence Typing, and genotypic resistances towards macrolides and quinolones based on point mutations in the 23S rRNA and gyrA genes, respectively, were determined. flaB-typing revealed 22 different types with one of them being novel and was useful to further differentiate strains with an identical Sequence Type (ST) and to identify a pigeon-specific clone. Macrolide resistance was not found, while quinolone resistance was detected in 23.3% of isolates. A relationship between specific genotypes and antibiotic resistance was observed, but was only significant for the Clonal Complex 206. Our data confirm that pigeons do not play a role in the spread of C. jejuni among cattle and they are not responsible for milk contamination. A relevant number of bulk milk samples were contaminated by C. jejuni resistant to quinolones, representing a possible source of human resistant strains.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Jonas, Romie; Kittl, Sonja and Kuhnert, Peter

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 630 Agriculture

ISSN:

1661-7827

Publisher:

Molecular Diversity Preservation International MDPI

Language:

English

Submitter:

Peter Kuhnert-Ryser

Date Deposited:

20 Nov 2014 08:11

Last Modified:

03 Mar 2015 15:33

Publisher DOI:

10.3390/ijerph110707154

PubMed ID:

25026083

BORIS DOI:

10.7892/boris.59966

URI:

https://boris.unibe.ch/id/eprint/59966

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