Cell force measurements in 3D microfabricated environments based on compliant cantilevers.

Marelli, Mattia; Gadhari, Neha; Boero, Giovanni; Chiquet, Matthias; Brugger, Jürgen (2014). Cell force measurements in 3D microfabricated environments based on compliant cantilevers. Lab on a chip, 14(2), pp. 286-293. Royal Society of Chemistry 10.1039/c3lc51021b

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We report the fabrication, functionalization and testing of microdevices for cell culture and cell traction force measurements in three-dimensions (3D). The devices are composed of bent cantilevers patterned with cell-adhesive spots not lying on the same plane, and thus suspending cells in 3D. The cantilevers are soft enough to undergo micrometric deflections when cells pull on them, allowing cell forces to be measured by means of optical microscopy. Since individual cantilevers are mechanically independent of each other, cell traction forces are determined directly from cantilever deflections. This proves the potential of these new devices as a tool for the quantification of cell mechanics in a system with well-defined 3D geometry and mechanical properties.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Orthodontics

UniBE Contributor:

Gadhari, Neha and Chiquet, Matthias

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1473-0197

Publisher:

Royal Society of Chemistry

Language:

English

Submitter:

Eveline Carmen Schuler

Date Deposited:

21 Nov 2014 08:19

Last Modified:

25 Jan 2017 12:15

Publisher DOI:

10.1039/c3lc51021b

PubMed ID:

24217771

BORIS DOI:

10.7892/boris.60251

URI:

https://boris.unibe.ch/id/eprint/60251

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