Proteomic analysis of porcine bone-conditioned medium.

Caballé-Serrano, Jordi; Bosshardt, Dieter; Buser, Daniel; Gruber, Reinhard (2014). Proteomic analysis of porcine bone-conditioned medium. International journal of oral & maxillofacial implants, 29(5), 1208-15d. Quintessence Publ. 10.11607/jomi.3708

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PURPOSE Autografts are considered to support bone regeneration. Paracrine factors released from cortical bone might contribute to the overall process of graft consolidation. The aim of this study was to characterize the paracrine factors by means of proteomic analysis. MATERIALS AND METHODS Bone-conditioned medium (BCM) was prepared from fresh bone chips of porcine mandibles and subjected to proteomic analysis. Proteins were categorized and clustered using the bioinformatic tools UNIPROT and PANTHER, respectively. RESULTS Proteomic analysis showed that BCM contains more than 150 proteins, of which 43 were categorized into "secreted" and "extracellular matrix." Growth factors that are not only detectable in BCM, but potentially also target cellular processes involved in bone regeneration, eg, pleiotrophin, galectin-1, transforming growth factor beta (TGF-β)-induced gene (TGFBI), lactotransferrin, insulin-like growth factor (IGF)-binding protein 5, latency-associated peptide forming a complex with TGF-β1, and TGF-β2, were discovered. CONCLUSION The present results demonstrate that cortical bone chips release a large spectrum of proteins with the possibility of modulating cellular aspects of bone regeneration. The data provide the basis for future studies to understand how these paracrine factors may contribute to the complex process of graft consolidation.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Oral Surgery Research
04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Periodontics Research
04 Faculty of Medicine > School of Dental Medicine > Department of Oral Surgery and Stomatology

UniBE Contributor:

Bosshardt, Dieter; Buser, Daniel and Gruber, Reinhard

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0882-2786

Publisher:

Quintessence Publ.

Language:

English

Submitter:

Eveline Carmen Schuler

Date Deposited:

02 Dec 2014 11:13

Last Modified:

25 Jan 2017 12:15

Publisher DOI:

10.11607/jomi.3708

PubMed ID:

25216150

Uncontrolled Keywords:

autograft, bone regeneration, conditioned medium, paracrine, proteomics, secretome, supernatant

BORIS DOI:

10.7892/boris.60636

URI:

https://boris.unibe.ch/id/eprint/60636

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