Electrochemical simulation of phase I metabolism for 21 drugs using four different working electrodes in an automated screening setup with MS detection.

Just Pedersen, Anders; Ambach, Lars; König, Stefan; Weinmann, Wolfgang (2014). Electrochemical simulation of phase I metabolism for 21 drugs using four different working electrodes in an automated screening setup with MS detection. Bioanalysis, 6(19), pp. 2607-2621. Future Science 10.4155/bio.14.166

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BACKGROUND

Electrochemical conversion of xenobiotics has been shown to mimic human phase I metabolism for a few compounds.

MATERIALS & METHODS

Twenty-one compounds were analyzed with a semiautomated electrochemical setup and mass spectrometry detection.

RESULTS

The system was able to mimic some metabolic pathways, such as oxygen gain, dealkylation and deiodination, but many of the expected and known metabolites were not produced.

CONCLUSION

Electrochemical conversion is a useful approach for the preparative synthesis of some types of metabolites, but as a screening method for unknown phase I metabolites, the method is, in our opinion, inferior to incubation with human liver microsomes and in vivo experiments with laboratory animals, for example.

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5 Citations in Web of Science ®
6 Citations in Scopus

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