Electrochemical simulation of phase I metabolism for 21 drugs using four different working electrodes in an automated screening setup with MS detection.

Just Pedersen, Anders; Ambach, Lars; König, Stefan; Weinmann, Wolfgang (2014). Electrochemical simulation of phase I metabolism for 21 drugs using four different working electrodes in an automated screening setup with MS detection. Bioanalysis, 6(19), pp. 2607-2621. Future Science 10.4155/bio.14.166

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BACKGROUND Electrochemical conversion of xenobiotics has been shown to mimic human phase I metabolism for a few compounds. MATERIALS & METHODS Twenty-one compounds were analyzed with a semiautomated electrochemical setup and mass spectrometry detection. RESULTS The system was able to mimic some metabolic pathways, such as oxygen gain, dealkylation and deiodination, but many of the expected and known metabolites were not produced. CONCLUSION Electrochemical conversion is a useful approach for the preparative synthesis of some types of metabolites, but as a screening method for unknown phase I metabolites, the method is, in our opinion, inferior to incubation with human liver microsomes and in vivo experiments with laboratory animals, for example.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute of Legal Medicine > Forensic Chemistry and Toxicology
04 Faculty of Medicine > Service Sector > Institute of Legal Medicine

UniBE Contributor:

Ambach, Lars; König, Stefan and Weinmann, Wolfgang

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1757-6180

Publisher:

Future Science

Language:

English

Submitter:

Antoinette Angehrn

Date Deposited:

03 Dec 2014 16:59

Last Modified:

06 Nov 2015 10:25

Publisher DOI:

10.4155/bio.14.166

PubMed ID:

25411704

BORIS DOI:

10.7892/boris.60692

URI:

https://boris.unibe.ch/id/eprint/60692

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