Identification of Clostridium chauvoei in cultures and clinical material from blackleg using PCR.

Kuhnert, Peter; Krampe, Marike; Capaul, Selja E.; Frey, Joachim; Nicolet, Jacques (1997). Identification of Clostridium chauvoei in cultures and clinical material from blackleg using PCR. Veterinary microbiology, 57(2-3), pp. 291-298. Elsevier 10.1016/S0378-1135(97)00129-6

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An identification system for Clostridium chauvoei, using PCR amplification of the 16S rRNA gene (rrs) with specific oligonucleotide primers and subsequent restriction digestion of the amplification product is described. The specific oligonucleotide primers were designed based on the rrs gene sequences of C. chauvoei by comparing it to the DNA sequences of the rrs genes of its most closely related species Clostridium septicum and Clostridium carnis. A subsequent restriction digestion of the 960 bp amplification product was used in order to unambiguously identify C. chauvoei. The developed identification system was evaluated on clinical material during a recent outbreak of blackleg in cattle. Thereby, C. chauvoei was identified as the etiologic agent of the outbreak either directly from clinical samples of muscle, liver, spleen and kidney or from primary cultures made with this material. A comparison of the newly developed method with standard diagnostic tools for C. chauvoei showed that it has advantages over the immunofluorescence and is, therefore, a useful option to it. Moreover, the assay is a valuable tool for the phylogenetic identification of C. chauvoei which can assist to substitute the fastidious traditional identification methods and replace laboratory animal testing currently used.

Item Type:

Journal Article (Further Contribution)

Division/Institute:

05 Veterinary Medicine > Other Institutions > Emeriti, Vetsuisse Faculty
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Kuhnert, Peter; Frey, Joachim and Nicolet, Jacques

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 630 Agriculture

ISSN:

0378-1135

Publisher:

Elsevier

Language:

English

Submitter:

Peter Kuhnert-Ryser

Date Deposited:

15 Dec 2014 12:02

Last Modified:

16 Dec 2014 15:26

Publisher DOI:

10.1016/S0378-1135(97)00129-6

PubMed ID:

9355263

BORIS DOI:

10.7892/boris.60714

URI:

https://boris.unibe.ch/id/eprint/60714

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