Comparative methodology to investigate the presence of Escherichia coli K-12 strains in environmental and human stool samples

Blum, G; Mühldorfer, I; Kuhnert, Peter; Frey, Joachim; Hacker, J (1996). Comparative methodology to investigate the presence of Escherichia coli K-12 strains in environmental and human stool samples. FEMS microbiology letters, 143(1), pp. 77-82. Wiley-Blackwell

Preview

Text 77.full.pdf - Published Version Available under License Publisher holds Copyright. Download (433kB) | Preview

This study was undertaken to evaluate the specificity and efficiency of different methods to detect Escherichia coli K-12 strains. Another aim was to determine the frequency of E. coli K-12 strains among wild-type E. coli isolates from different sources. The detection of K-12 strains was performed both genotypically by K-12 specific polymerase chain reaction (PCR) and on the basis of phenotypical tests. In addition, the genome structures of E. coli strains were characterized by pulsed-field gel electrophoresis (PFGE). The most specific results could be obtained by the genotypical tests PCR and PFGE as well as by the K-12 specific phage assay. In total, 131 stool and 95 water isolates as well as 14 K-12 derivatives were examined by the different methods. No E. coli K-12 strains were detected among the wild-type isolates.

Interest & Impact

Downloads

28 since deposited on 06 Feb 2015
17 in the past 12 months

Citations

5 Citations in Web of Science ®
6 Citations in Scopus

Search

in Google Scholar™

Services

Actions (login required)

Edit item

Actions (login required)

Edit item