Comparative methodology to investigate the presence of Escherichia coli K-12 strains in environmental and human stool samples

Blum, G; Mühldorfer, I; Kuhnert, Peter; Frey, Joachim; Hacker, J (1996). Comparative methodology to investigate the presence of Escherichia coli K-12 strains in environmental and human stool samples. FEMS microbiology letters, 143(1), pp. 77-82. Wiley-Blackwell

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This study was undertaken to evaluate the specificity and efficiency of different methods to detect Escherichia coli K-12 strains. Another aim was to determine the frequency of E. coli K-12 strains among wild-type E. coli isolates from different sources. The detection of K-12 strains was performed both genotypically by K-12 specific polymerase chain reaction (PCR) and on the basis of phenotypical tests. In addition, the genome structures of E. coli strains were characterized by pulsed-field gel electrophoresis (PFGE). The most specific results could be obtained by the genotypical tests PCR and PFGE as well as by the K-12 specific phage assay. In total, 131 stool and 95 water isolates as well as 14 K-12 derivatives were examined by the different methods. No E. coli K-12 strains were detected among the wild-type isolates.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Kuhnert, Peter and Frey, Joachim

Subjects:

600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology

ISSN:

0378-1097

Publisher:

Wiley-Blackwell

Language:

English

Submitter:

Peter Kuhnert-Ryser

Date Deposited:

06 Feb 2015 10:20

Last Modified:

07 Feb 2015 00:56

PubMed ID:

8807805

BORIS DOI:

10.7892/boris.60720

URI:

https://boris.unibe.ch/id/eprint/60720

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