Remote Effects Modulating the Spin Equilibrium of the Resting State of Cytochrome P450cam –An Investigation Using Active Site Analogues

Lochner, Martin; Mu, Linjing; Woggon, Wolf-D. (2003). Remote Effects Modulating the Spin Equilibrium of the Resting State of Cytochrome P450cam –An Investigation Using Active Site Analogues. Advanced synthesis & catalysis, 345(6-7), pp. 743-765. Wiley-VCH 10.1002/adsc.200303011

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The crystal structure of the resting state of cytochrome P450cam (CYP101), a heme thiolate protein, shows a cluster of six water molecules in the substrate binding pocket, one of which is coordinating to iron(III) as sixth ligand. The resting state is low-spin and changes to high-spin when substrate camphor binds and H2O is removed. In contrast to the protein, previously synthesised enzyme models such as H2O[BOND]FeIII(porph)(ArS−) were shown to be purely high-spin. Iron(S−)porphyrins with different distal sites mimicking proposed remote effects have been prepared and studied by cw-EPR. The results indicate that the low-spin of the resting state of P450cam is due to the fact that the water molecule coordinating to iron has an OH−-like character because of hydrogen bonding and polarisation of the water cluster, respectively.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Departement of Chemistry and Biochemistry

UniBE Contributor:

Lochner, Martin

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry

ISSN:

1615-4150

Publisher:

Wiley-VCH

Language:

English

Submitter:

Martin Lochner

Date Deposited:

19 Dec 2014 10:51

Last Modified:

19 Dec 2014 10:51

Publisher DOI:

10.1002/adsc.200303011

BORIS DOI:

10.7892/boris.60960

URI:

https://boris.unibe.ch/id/eprint/60960

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