Activation of intervertebral disc cells by co-culture with notochordal cells, conditioned medium and hypoxia

Gantenbein, Benjamin; Calandriello, Elena; Wuertz-Kozak, Karin; Benneker, Lorin Michael; Keel, Marius JB; Chan, Samantha CW (2014). Activation of intervertebral disc cells by co-culture with notochordal cells, conditioned medium and hypoxia. BMC musculoskeletal disorders, 15(422), p. 422. BioMed Central 10.1186/1471-2474-15-422

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Notochordal cells (NC) remain in the focus of research for regenerative therapy for the degenerated intervertebral disc (IVD) due to their progenitor status. Recent findings suggested their regenerative action on more mature disc cells, presumably by the secretion of specific factors, which has been described as notochordal cell conditioned medium (NCCM). The aim of this study was to determine NC culture conditions (2D/3D, fetal calf serum, oxygen level) that lead to significant IVD cell activation in an indirect co-culture system under normoxia and hypoxia (2% oxygen).

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Orthopaedic, Plastic and Hand Surgery (DOPH) > Clinic of Orthopaedic Surgery
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute for Surgical Technology & Biomechanics ISTB

UniBE Contributor:

Gantenbein, Benjamin and Benneker, Lorin Michael

Subjects:

600 Technology > 610 Medicine & health
500 Science > 570 Life sciences; biology
600 Technology > 620 Engineering

ISSN:

1471-2474

Publisher:

BioMed Central

Language:

English

Submitter:

Benjamin Gantenbein

Date Deposited:

05 Jan 2015 16:27

Last Modified:

26 Jun 2016 01:56

Publisher DOI:

10.1186/1471-2474-15-422

PubMed ID:

25496082

BORIS DOI:

10.7892/boris.61154

URI:

https://boris.unibe.ch/id/eprint/61154

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