Engraftment of autologous bone marrow cells into the injured cranial cruciate ligament in dogs

Linon, Elisa; Spreng, David; Rytz, Ulrich; Forterre, Simone (2014). Engraftment of autologous bone marrow cells into the injured cranial cruciate ligament in dogs. Veterinary journal, 202(3), pp. 448-454. Elsevier 10.1016/j.tvjl.2014.08.031

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Current research indicates that exogenous stem cells may accelerate reparative processes in joint disease but, no previous studies have evaluated whether bone marrow cells (BMCs) target the injured cranial cruciate ligament (CCL) in dogs. The objective of this study was to investigate engraftment of BMCs following intra-articular injection in dogs with spontaneous CCL injury. Autologous PKH26-labelled BMCs were injected into the stifle joint of eight client-owned dogs with CCL rupture. The effects of PKH26 staining on cell viability and PKH26 fluorescence intensity were analysed in vitro using a MTT assay and flow cytometry. Labelled BMCs in injured CCL tissue were identified using fluorescence microscopy of biopsies harvested 3 and 13 days after intra-articular BMC injection. The intensity of PKH26 fluorescence declines with cell division but was still detectable after 16 days. Labelling with PKH26 had no detectable effect on cell viability or proliferation. Only rare PKH26-positive cells were present in biopsies of the injured CCL in 3/7 dogs and in synovial fluid in 1/7 dogs. No differences in transforming growth factor-beta1, and interleukin-6 before and after BMC treatment were found and no clinical complications were noted during a 1 year follow-up period. In conclusion, BMCs were shown to engraft to the injured CCL in dogs when injected into the articular cavity. Intra-articular application of PKH26-labelled cultured mesenchymal stem cells is likely to result in higher numbers of engrafted cells that can be tracked using this method in a clinical setting.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV)
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Small Animal Clinic > Small Animal Clinic, Surgery
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Small Animal Clinic

UniBE Contributor:

Linon, Elisa; Spreng, David; Rytz, Ulrich and Forterre, Simone

Subjects:

500 Science
600 Technology > 610 Medicine & health

ISSN:

1090-0233

Publisher:

Elsevier

Language:

English

Submitter:

Simone Forterre

Date Deposited:

02 Feb 2015 15:21

Last Modified:

12 Sep 2017 02:26

Publisher DOI:

10.1016/j.tvjl.2014.08.031

PubMed ID:

25261229

BORIS DOI:

10.7892/boris.61314

URI:

https://boris.unibe.ch/id/eprint/61314

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