Emdogain-regulated gene expression in palatal fibroblasts requires TGF-βRI kinase signaling.

Stähli, Alexandra Beatrice; Bosshardt, Dieter; Sculean, Anton; Gruber, Reinhard (2014). Emdogain-regulated gene expression in palatal fibroblasts requires TGF-βRI kinase signaling. PLoS ONE, 9(9), e105672. Public Library of Science 10.1371/journal.pone.0105672

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Genome-wide microarrays have suggested that Emdogain regulates TGF-β target genes in gingival and palatal fibroblasts. However, definitive support for this contention and the extent to which TGF-β signaling contributes to the effects of Emdogain has remained elusive. We therefore studied the role of the TGF-β receptor I (TGF-βRI) kinase to mediate the effect of Emdogain on palatal fibroblasts. Palatal fibroblasts were exposed to Emdogain with and without the inhibitor for TGF-βRI kinase, SB431542. Emdogain caused 39 coding genes to be differentially expressed in palatal fibroblasts by microarray analysis (p<0.05; >10-fold). Importantly, in the presence of the TGF-βRI kinase inhibitor SB431542, Emdogain failed to cause any significant changes in gene expression. Consistent with this mechanism, three independent TGF-βRI kinase inhibitors and a TGF-β neutralizing antibody abrogated the increased expression of IL-11, a selected Emdogain target gene. The MAPK inhibitors SB203580 and U0126 lowered the impact of Emdogain on IL-11 expression. The data support that TGF-βRI kinase activity is necessary to mediate the effects of Emdogain on gene expression in vitro.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology

UniBE Contributor:

Stähli, Alexandra Beatrice; Bosshardt, Dieter; Sculean, Anton and Gruber, Reinhard

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1932-6203

Publisher:

Public Library of Science

Language:

English

Submitter:

Eveline Carmen Schuler

Date Deposited:

07 Jan 2015 09:19

Last Modified:

25 Jan 2017 12:15

Publisher DOI:

10.1371/journal.pone.0105672

PubMed ID:

25197981

BORIS DOI:

10.7892/boris.61504

URI:

https://boris.unibe.ch/id/eprint/61504

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