Biomechanical loading modulates proinflammatory and bone resorptive mediators in bacterial-stimulated PDL cells.

Nogueira, Andressa Vilas Boas; Nokhbehsaim, Marjan; Eick, Sigrun; Bourauel, Christoph; Jäger, Andreas; Jepsen, Søren; Rossa, Carlos; Deschner, James; Cirelli, Joni Augusto (2014). Biomechanical loading modulates proinflammatory and bone resorptive mediators in bacterial-stimulated PDL cells. Mediators of inflammation, 2014, p. 425421. Hindawi Publishing Corp. 10.1155/2014/425421

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The present study aimed to evaluate in vitro whether biomechanical loading modulates proinflammatory and bone remodeling mediators production by periodontal ligament (PDL) cells in the presence of bacterial challenge. Cells were seeded on BioFlex culture plates and exposed to Fusobacterium nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL) and high (CTSH) magnitudes for 1 and 3 days. Synthesis of cyclooxygenase-2 (COX2) and prostaglandin E2 (PGE2) was evaluated by ELISA. Gene expression and protein secretion of osteoprotegerin (OPG) and receptor activator of nuclear factor kappa-B ligand (RANKL) were evaluated by quantitative RT-PCR and ELISA, respectively. F. nucleatum increased the production of COX2 and PGE2, which was further increased by CTS. F. nucleatum-induced increase of PGE2 synthesis was significantly (P < 0.05) increased when CTSH was applied at 1 and 3 days. In addition, CTSH inhibited the F. nucleatum-induced upregulation of OPG at 1 and 3 days, thereby increasing the RANKL/OPG ratio. OPG and RANKL mRNA results correlated with the protein results. In summary, our findings provide original evidence that CTS can enhance bacterial-induced syntheses of molecules associated with inflammation and bone resorption by PDL cells. Therefore, biomechanical, such as orthodontic or occlusal, loading may enhance the bacterial-induced inflammation and destruction in periodontitis.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Periodontics Research
04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology

UniBE Contributor:

Eick, Sigrun

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0962-9351

Publisher:

Hindawi Publishing Corp.

Language:

English

Submitter:

Eveline Carmen Schuler

Date Deposited:

13 Jan 2015 17:11

Last Modified:

25 Jan 2017 12:15

Publisher DOI:

10.1155/2014/425421

PubMed ID:

24976684

BORIS DOI:

10.7892/boris.61668

URI:

https://boris.unibe.ch/id/eprint/61668

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