Honey - a potential agent against Porphyromonas gingivalis: an in vitro study.

Eick, Sigrun; Schäfer, Gesine; Kwieciński, Jakub; Atrott, Julia; Henle, Thomas; Pfister, Wolfgang (2014). Honey - a potential agent against Porphyromonas gingivalis: an in vitro study. BMC Oral Health, 14, p. 24. BioMed Central 10.1186/1472-6831-14-24

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BACKGROUND

Honey has been discussed as a therapeutic option in wound healing since ancient time. It might be also an alternative to the commonly used antimicrobials in periodontitis treatment. The in-vitro study was aimed to determine the antimicrobial efficacy against Porphyromonas gingivalis as a major periodontopathogen.

METHODS

One Manuka and one domestic beekeeper honey have been selected for the study. As a screening, MICs of the honeys against 20 P. gingivalis strains were determined. Contents of methylglyoxal and hydrogen peroxide as the potential antimicrobial compounds were determined. These components (up to 100 mg/l), propolis (up to 200 mg/l) as well as the two honeys (up to 10% w/v) were tested against four P. gingivalis strains in planktonic growth and in a single-species biofilm.

RESULTS

2% of Manuka honey inhibited the growth of 50% of the planktonic P. gingivalis, the respective MIC50 of the German beekeeper honey was 5%. Manuka honey contained 1.87 mg/kg hydrogen peroxide and the domestic honey 3.74 mg/kg. The amount of methylglyoxal was found to be 2 mg/kg in the domestic honey and 982 mg/kg in the Manuka honey. MICs for hydrogen peroxide were 10 mg/l - 100 mg/l, for methylglyoxal 5 - 20 mg/l, and for propolis 20 mg/l - 200 mg/l. 10% of both types of honey inhibited the formation of P. gingivalis biofilms and reduced the numbers of viable bacteria within 42 h-old biofilms. Neither a total prevention of biofilm formation nor a complete eradication of a 42 h-old biofilm by any of the tested compounds and the honeys were found.

CONCLUSIONS

Honey acts antibacterial against P. gingivalis. The observed pronounced effects of Manuka honey against planktonic bacteria but not within biofilm can be attributed to methylglyoxal as the characteristic antimicrobial component.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology

UniBE Contributor:

Eick, Sigrun

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1472-6831

Publisher:

BioMed Central

Language:

English

Submitter:

Eveline Carmen Schuler

Date Deposited:

19 Jan 2015 16:49

Last Modified:

02 Sep 2020 08:23

Publisher DOI:

10.1186/1472-6831-14-24

PubMed ID:

24666777

Uncontrolled Keywords:

Honey, Porphyromonas gingivalis, Biofilm, Methylglyoxal, Minimal inhibitory concentration

BORIS DOI:

10.7892/boris.61671

URI:

https://boris.unibe.ch/id/eprint/61671

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