Subgingival microbiota of Sri Lankan tea labourers naïve to oral hygiene measures.

Zhuang, Long-Fei; Watt, Rory M; Steiner, Sarah; Lang-Hua, Bich Hue; Wang, Ren; Ramseier, Christoph Andreas; Lang, Niklaus Peter (2014). Subgingival microbiota of Sri Lankan tea labourers naïve to oral hygiene measures. Journal of clinical periodontology, 41(5), pp. 433-441. Wiley 10.1111/jcpe.12230

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AIM To characterize the subgingival microbiota within a cohort of adult males (n = 32) naïve to oral hygiene practices, and to compare the composition of bacterial taxa present in periodontal sites with various probing depths. MATERIAL AND METHODS Subgingival plaque samples were collected from single shallow pocket [pocket probing depth (PPD)≤3 mm] and deep pocket (PPD≥6 mm) sites from each subject. A polymerase chain reaction based strategy was used to construct a clone library of 16S ribosomal RNA (rRNA) genes for each site. The sequences of ca. 30-60 plasmid clones were determined for each site to identify resident taxa. Microbial composition was compared using a variety of statistical and bioinformatics approaches. RESULTS A total of 1887 cloned 16S rRNA gene sequences were analysed, which were assigned to 318 operational taxonomic units (98% identity cut-off). The subgingival microbiota was dominated by Firmicutes (69.8%), Proteobacteria (16.3%), and Fusobacteria (8.0%). The overall composition of microbial communities in shallow sites was significantly different from those within deep sites (∫-Libshuff, p < 0.001). CONCLUSIONS A taxonomically diverse subgingival microbiota was present within this cohort; however, the structures of the microbial communities present in the respective subjects exhibited limited variation. Deep and shallow sites contained notably different microbial compositions, but this was not correlated with the rate of periodontal progression.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology

UniBE Contributor:

Ramseier, Christoph Andreas and Lang, Niklaus Peter

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0303-6979

Publisher:

Wiley

Language:

English

Submitter:

Eveline Carmen Schuler

Date Deposited:

15 Jan 2015 09:26

Last Modified:

25 Jan 2017 12:15

Publisher DOI:

10.1111/jcpe.12230

PubMed ID:

24460707

Uncontrolled Keywords:

disease aetiology, microbial ecology, oral hygiene, periodontitis, subgingival plaque

BORIS DOI:

10.7892/boris.61681

URI:

https://boris.unibe.ch/id/eprint/61681

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