Microbial glycan microarrays define key features of host-microbial interactions

Stowell, Sean R.; Arthur, Connie M.; Mc Bride, Ryan; Berger, Oren; Razi, Nahid; Heimberg-Molinaro, Jamie; Rodrigues, Lilian C.; Gourdine, Jean-Philippe; Noll, Alexander J.; von Gunten, Stephan; Smith, David F.; Knirel, Yuriy A.; Paulson, James C.; Cummings, Richard D. (2014). Microbial glycan microarrays define key features of host-microbial interactions. Nature Chemical Biology, 10(6), pp. 470-476. Nature Publishing Group 10.1038/nchembio.1525

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Genomic approaches continue to provide unprecedented insight into the microbiome, yet host immune interactions with diverse microbiota can be difficult to study. We therefore generated a microbial microarray containing defined antigens isolated from a broad range of microbial flora to examine adaptive and innate immunity. Serological studies with this microarray show that immunoglobulins from multiple mammalian species have unique patterns of reactivity, whereas exposure of animals to distinct microbes induces specific serological recognition. Although adaptive immunity exhibited plasticity toward microbial antigens, immunological tolerance limits reactivity toward self. We discovered that several innate immune galectins show specific recognition of microbes that express self-like antigens, leading to direct killing of a broad range of Gram-negative and Gram-positive microbes. Thus, host protection against microbes seems to represent a balance between adaptive and innate immunity to defend against evolving antigenic determinants while protecting against molecular mimicry.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Pharmacology

UniBE Contributor:

von Gunten, Stephan

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1552-4450

Publisher:

Nature Publishing Group

Language:

English

Submitter:

Anita Dähler

Date Deposited:

19 Jan 2015 15:20

Last Modified:

26 Jun 2016 01:57

Publisher DOI:

10.1038/nchembio.1525

PubMed ID:

24814672

BORIS DOI:

10.7892/boris.62040

URI:

https://boris.unibe.ch/id/eprint/62040

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