International Clostridium difficile animal strain collection and large diversity of animal associated strains

Janezic, Sandra; Zidaric, Valerija; Pardon, Bart; Indra, Alexander; Kokotovic, Branko; Blanco, Jose Luis; Seyboldt, Christian; Diaz, Cristina Rodriguez; Poxton, Ian R; Perreten, Vincent; Drigo, Ilenia; Jiraskova, Alena; Ocepek, Matjaz; Weese, J Scott; Songer, J Glenn; Wilcox, Mark H; Rupnik, Maja (2014). International Clostridium difficile animal strain collection and large diversity of animal associated strains. BMC microbiology, 14, p. 173. BioMed Central 10.1186/1471-2180-14-173

[img]
Preview
Text
1471-2180-14-173.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (1MB) | Preview

BACKGROUND Clostridium difficile is an important cause of intestinal infections in some animal species and animals might be a reservoir for community associated human infections. Here we describe a collection of animal associated C. difficile strains from 12 countries based on inclusion criteria of one strain (PCR ribotype) per animal species per laboratory. RESULTS Altogether 112 isolates were collected and distributed into 38 PCR ribotypes with agarose based approach and 50 PCR ribotypes with sequencer based approach. Four PCR ribotypes were most prevalent in terms of number of isolates as well as in terms of number of different host species: 078 (14.3% of isolates; 4 hosts), 014/020 (11.6%; 8 hosts); 002 (5.4%; 4 hosts) and 012 (5.4%; 5 hosts). Two animal hosts were best represented; cattle with 31 isolates (20 PCR ribotypes; 7 countries) and pigs with 31 isolates (16 PCR ribotypes; 10 countries). CONCLUSIONS This results show that although PCR ribotype 078 is often reported as the major animal C. difficile type, especially in pigs, the variability of strains in pigs and other animal hosts is substantial. Most common human PCR ribotypes (014/020 and 002) are also among most prevalent animal associated C. difficile strains worldwide. The widespread dissemination of toxigenic C. difficile and the considerable overlap in strain distribution between species furthers concerns about interspecies, including zoonotic, transmission of this critically important pathogen.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Perreten, Vincent

Subjects:

600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1471-2180

Publisher:

BioMed Central

Language:

English

Submitter:

Vincent Perreten

Date Deposited:

06 Feb 2015 09:54

Last Modified:

06 Mar 2015 16:06

Publisher DOI:

10.1186/1471-2180-14-173

PubMed ID:

24972659

BORIS DOI:

10.7892/boris.62546

URI:

https://boris.unibe.ch/id/eprint/62546

Actions (login required)

Edit item Edit item
Provide Feedback