Improved bioassay for the detection of porcine tumor necrosis factor using a homologous cell line: PK(15)

Bertoni, Giuseppe; Kuhnert, Peter; Peterhans, Ernst; Pauli, U (1993). Improved bioassay for the detection of porcine tumor necrosis factor using a homologous cell line: PK(15). Journal of immunological methods, 160(2), pp. 267-271. Elsevier

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Close similarities of various physiological parameters makes the pig one of the preferred animal models for the study of human diseases, especially those involving the cardiovascular system. Unfortunately, the use of pig models to study diseases such as viral hemorrhagic fevers and endotoxic shock syndrome have been hampered by the lack of the necessary immunological tools to measure important immunoregulatory cytokines such as tumor necrosis factor (TNF). Here we describe a TNF-bioassay which is based on the porcine kidney cell line PK(15). Compared to the widely used murine fibroblastoid cell line L929, the PK(15) cell line displays a 100-1000-fold higher sensitivity for porcine TNF-alpha, a higher sensitivity for human TNF-alpha, and a slightly lower sensitivity for murine TNF-alpha. Using a PK(15) bioassay we can detect recombinant TNF-alpha as well as cytotoxic activity in the supernatants of lipopolysaccharide (LPS)-activated porcine monocytes at high dilutions. This suggests that the sensitivity of the test should permit the detection of TNF in biological specimens such as pig serum.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Other Institutions > Emeriti, Vetsuisse Faculty

UniBE Contributor:

Bertoni, Giuseppe; Kuhnert, Peter and Peterhans, Ernst

Subjects:

600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology

ISSN:

0022-1759

Publisher:

Elsevier

Language:

English

Submitter:

Peter Kuhnert-Ryser

Date Deposited:

09 Feb 2015 12:58

Last Modified:

09 Feb 2015 12:58

PubMed ID:

8459114

BORIS DOI:

10.7892/boris.62558

URI:

https://boris.unibe.ch/id/eprint/62558

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