miR-21 functionally interacts with the 3'UTR of chemokine CCL20 and down-regulates CCL20 expression in miR-21 transfected colorectal cancer cells

Vicinus, Benjamin; Rubie, Claudia; Faust, Sabrina K; Frick, Vilma Oliveira; Ghadjar, Pirus; Wagner, Mathias; Graeber, Stefan; Schilling, Martin K (2012). miR-21 functionally interacts with the 3'UTR of chemokine CCL20 and down-regulates CCL20 expression in miR-21 transfected colorectal cancer cells. Cancer letters, 316(1), pp. 105-12. Amsterdam: Elsevier 10.1016/j.canlet.2011.10.031

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As deregulation of miRNAs and chemokine CCL20 was shown to play a role in colorectal cancer (CRC) pathogenesis, we analyzed the functional interactions of candidate miRNAs with CCL20 mRNA. After target prediction software programs indicated a role for miR-21 in CCL20 regulation, we applied the luciferase reporter assay system to demonstrate that miR-21 functionally interacts with the 3'UTR of CCL20 mRNA and down-regulates CCL20 in miR-21 mimic transfected CRC cell lines (Caco-2, SW480 and SW620). Thus, regulation of CCL20 expression by miR-21 might be a regulatory mechanism involved in progression of CRC.

Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Clinic of Radiation Oncology
UniBE Contributor:	Ghadjar, Pirus
ISSN:	0304-3835
Publisher:	Elsevier
Language:	English
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 14:19
Last Modified:	05 Dec 2022 14:05
Publisher DOI:	10.1016/j.canlet.2011.10.031
PubMed ID:	22099878
Web of Science ID:	000299712400015
URI:	https://boris.unibe.ch/id/eprint/6256 (FactScience: 211166)