Genomic heterogeneity of small ruminant lentiviruses detected by PCR

Zanoni, Reto Giacomo; Nauta, I M; Kuhnert, Peter; Pauli, U; Pohl, B; Peterhans, Ernst (1992). Genomic heterogeneity of small ruminant lentiviruses detected by PCR. Veterinary microbiology, 33(1-4), pp. 341-351. Elsevier

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In order to detect a large spectrum of small ruminant lentiviruses, primers for PCR were chosen in conserved parts of the LTR and GAG genes of Icelandic Visna virus 1514 and of the POL gene of caprine arthritis-encephalitis virus. This set of primers was tested in six different caprine arthritis-encephalitis virus (CAEV)- and Maedi-Visna virus isolates of Dutch, American and Swiss origin. The LTR primers allowed the detection of the corresponding fragments of all isolates. The GAG primers allowed amplification of the corresponding fragments of all but the Swiss Maedi-Visna virus strain OLV. Using the POL primers, one Maedi-Visna- and two caprine arthritis-encephalitis virus strains were detected after one round of amplification. Sequencing of the GAG and POL amplification products and comparison to Icelandic Visna virus and CAEV strain CO revealed total heterogeneity of 38% for the GAG- and 28% for the POL fragment. The virus strains studied fall into two groups which are more closely related to one another than to Icelandic Visna virus.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Other Institutions > Emeriti, Vetsuisse Faculty

UniBE Contributor:

Zanoni, Reto Giacomo; Kuhnert, Peter and Peterhans, Ernst

Subjects:

600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology

ISSN:

0378-1135

Publisher:

Elsevier

Language:

English

Submitter:

Peter Kuhnert-Ryser

Date Deposited:

09 Feb 2015 13:08

Last Modified:

09 Feb 2015 13:08

PubMed ID:

1336245

URI:

https://boris.unibe.ch/id/eprint/62561

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