The porcine tumor necrosis factor-encoding genes: sequence and comparative analysis

Kuhnert, Peter; Wüthrich, C; Peterhans, Ernst; Pauli, U (1991). The porcine tumor necrosis factor-encoding genes: sequence and comparative analysis. Gene, 102(2), pp. 171-178. Elsevier

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We have cloned and sequenced a 10.22-kb fragment of the genomic locus of the porcine tumor necrosis factor-encoding genes, TNF-alpha and TNF-beta. A liver genomic DNA library, partially digested with Sau3AI, was cloned into the phage lambda EMBL4 and screened with a porcine TNF-alpha cDNA probe. Analysis showed that both the TNF-alpha and TNF-beta genes were present on the cloned fragment. In addition, the cloned fragment contained about 2 kb of repetitive sequences 5' to the TNF-beta gene. The TNF genes are arranged in a tandem repeat, as is the case for the human, mouse and rabbit TNF genes. The comparison of both genes with their human homologues displayed a considerable degree of conservation (80%), suggesting an equal evolution rate.

Item Type:	Journal Article (Original Article)
Division/Institute:	05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction 05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology 05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) 05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology
UniBE Contributor:	Kuhnert, Peter, Peterhans, Ernst
Subjects:	500 Science > 570 Life sciences; biology 600 Technology > 630 Agriculture
ISSN:	0378-1119
Publisher:	Elsevier
Language:	English
Submitter:	Peter Kuhnert-Ryser
Date Deposited:	14 Sep 2015 16:49
Last Modified:	05 Dec 2022 14:39
PubMed ID:	1874444
URI:	https://boris.unibe.ch/id/eprint/62565