A genomic perspective on a new bacterial genus and species from the Alcaligenaceae family, Basilea psittacipulmonis

Whiteson, Katrine L; Hernandez, David; Lazarevic, Vladimir; Gaia, Nadia; Farinelli, Laurent; François, Patrice; Pilo, Paola; Frey, Joachim; Schrenzel, Jacques (2014). A genomic perspective on a new bacterial genus and species from the Alcaligenaceae family, Basilea psittacipulmonis. BMC Genomics, 15, p. 169. BioMed Central 10.1186/1471-2164-15-169

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BACKGROUND A novel Gram-negative, non-haemolytic, non-motile, rod-shaped bacterium was discovered in the lungs of a dead parakeet (Melopsittacus undulatus) that was kept in captivity in a petshop in Basel, Switzerland. The organism is described with a chemotaxonomic profile and the nearly complete genome sequence obtained through the assembly of short sequence reads. RESULTS Genome sequence analysis and characterization of respiratory quinones, fatty acids, polar lipids, and biochemical phenotype is presented here. Comparison of gene sequences revealed that the most similar species is Pelistega europaea, with BLAST identities of only 93% to the 16S rDNA gene, 76% identity to the rpoB gene, and a similar GC content (~43%) as the organism isolated from the parakeet, DSM 24701 (40%). The closest full genome sequences are those of Bordetella spp. and Taylorella spp. High-throughput sequencing reads from the Illumina-Solexa platform were assembled with the Edena de novo assembler to form 195 contigs comprising the ~2 Mb genome. Genome annotation with RAST, construction of phylogenetic trees with the 16S rDNA (rrs) gene sequence and the rpoB gene, and phylogenetic placement using other highly conserved marker genes with ML Tree all suggest that the bacterial species belongs to the Alcaligenaceae family. Analysis of samples from cages with healthy parakeets suggested that the newly discovered bacterial species is not widespread in parakeet living quarters. CONCLUSIONS Classification of this organism in the current taxonomy system requires the formation of a new genus and species. We designate the new genus Basilea and the new species psittacipulmonis. The type strain of Basilea psittacipulmonis is DSM 24701 (= CIP 110308 T, 16S rDNA gene sequence Genbank accession number JX412111 and GI 406042063).

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Pilo, Paola and Frey, Joachim

Subjects:

600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology

ISSN:

1471-2164

Publisher:

BioMed Central

Language:

English

Submitter:

Amandine Ruffieux

Date Deposited:

09 Feb 2015 14:41

Last Modified:

17 Aug 2018 12:02

Publisher DOI:

10.1186/1471-2164-15-169

PubMed ID:

24581117

BORIS DOI:

10.7892/boris.62660

URI:

https://boris.unibe.ch/id/eprint/62660

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