Bacteriological diagnosis and molecular strain typing of Mycobacterium bovis and Mycobacterium caprae

Gormley, E; Corner, L A L; Costello, E; Rodriguez-Campos, Sabrina (2014). Bacteriological diagnosis and molecular strain typing of Mycobacterium bovis and Mycobacterium caprae. Research in veterinary science, 97 Suppl, S30-43. Association for Veterinary Teaching and Research Work 10.1016/j.rvsc.2014.04.010

[img] Text
Gormley2014.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (872kB) | Request a copy

The primary isolation of a Mycobacterium sp. of the Mycobacterium tuberculosis complex from an infected animal provides a definitive diagnosis of tuberculosis. However, as Mycobacterium bovis and Mycobacterium caprae are difficult to isolate, particularly for animals in the early stages of disease, success is dependent on the optimal performance of all aspects of the bacteriological process, from the initial choice of tissue samples at post-mortem examination or clinical samples, to the type of media and conditions used to cultivate the microorganism. Each step has its own performance characteristics, which can contribute to sensitivity and specificity of the procedure, and may need to be optimized in order to achieve the gold standard diagnosis. Having isolated the slow-growing mycobacteria, species identification and fine resolution strain typing are keys to understanding the epidemiology of the disease and to devise strategies to limit transmission of infection. New technologies have emerged that can now even discriminate different isolates from the same animal. In this review we highlight the key factors that contribute to the accuracy of bacteriological diagnosis of M. bovis and M. caprae, and describe the development of advanced genotyping techniques that are increasingly used in diagnostic laboratories for the purpose of supporting detailed epidemiological investigations.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Rodriguez, Sabrina

Subjects:

600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology

ISSN:

0034-5288

Publisher:

Association for Veterinary Teaching and Research Work

Language:

English

Submitter:

Sabrina Rodriguez Campos

Date Deposited:

09 Feb 2015 16:30

Last Modified:

26 Jun 2016 01:58

Publisher DOI:

10.1016/j.rvsc.2014.04.010

PubMed ID:

24833269

Uncontrolled Keywords:

Mycobacterium bovis, M. caprae, Tuberculosis, Culture, Diagnosis, Strain typing, Spoligotype, VNTR

BORIS DOI:

10.7892/boris.62670

URI:

https://boris.unibe.ch/id/eprint/62670

Actions (login required)

Edit item Edit item
Provide Feedback