S100A1 and S100B expression patterns identify differentiation status of human articular chondrocytes.

Diaz Romero, Jose; Quintin, Aurelie; Schoenholzer, Eric; Pauli, Chantal; Despont, Alain; Zumstein, Matthias; Kohl, Sandro; Nesic, Dobrila (2014). S100A1 and S100B expression patterns identify differentiation status of human articular chondrocytes. Journal of cellular physiology, 229(8), pp. 1106-1117. Wiley 10.1002/jcp.24547

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Many studies in the field of cell-based cartilage repair have focused on identifying markers associated with the differentiation status of human articular chondrocytes (HAC) that could predict their chondrogenic potency. A previous study from our group showed a correlation between the expression of S100 protein in HAC and their chondrogenic potential. The aims of the current study were to clarify which S100 proteins are associated with HAC differentiation status and to provide an S100-based assay for measuring HAC chondrogenic potential. The expression patterns of S100A1 and S100B were investigated in cartilage and in HAC cultured under conditions promoting dedifferentiation (monolayer culture) or redifferentiation (pellet culture or BMP4 treatment in monolayer culture), using characterized antibodies specifically recognizing S100A1 and S100B, by immunohistochemistry, immunocytochemistry, Western blot, and gene expression analysis. S100A1 and S100B were expressed homogeneously in all cartilage zones, and decreased during dedifferentiation. S100A1, but not S100B, was re-expressed in pellets and co-localized with collagen II. Gene expression analysis revealed concomitant modulation of S100A1, S100B, collagen type II, and aggrecan: down-regulation during monolayer culture and up-regulation upon BMP4 treatment. These results strongly support an association of S100A1, and to a lesser extent S100B, with the HAC differentiated phenotype. To facilitate their potential application, we established an S100A1/B-based flow cytometry assay for accurate assessment of HAC differentiation status. We propose S100A1 and S100B expression as a marker to develop potency assays for cartilage regeneration cell therapies, and as a redifferentiation readout in monolayer cultures aiming to investigate stimuli for chondrogenic induction.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Orthopaedic, Plastic and Hand Surgery (DOPH) > Clinic of Orthopaedic Surgery
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Knochenbiologie & Orthopädische Forschung
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Knochenbiologie & Orthopädische Forschung

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Herz und Gefässe

UniBE Contributor:

Diaz Romero, Jose; Quintin, Aurelie; Despont, Alain; Zumstein, Matthias; Kohl, Sandro and Nesic, Dobrila

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0021-9541

Publisher:

Wiley

Language:

English

Submitter:

Verena de Serra Frazao-Bill

Date Deposited:

11 Feb 2015 10:40

Last Modified:

02 Nov 2015 11:01

Publisher DOI:

10.1002/jcp.24547

PubMed ID:

24402969

URI:

https://boris.unibe.ch/id/eprint/62951

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