Immunoblotting for the serodiagnosis of alveolar echinococcosis in alive and dead Eurasian beavers (Castor fiber)

Gottstein, Bruno; Frey, Caroline; Campbell-Palmer, R; Pizzi, R; Barlow, A; Hentrich, Brigitte; Posautz, A; Ryser-Degiorgis, Marie-Pierre (2014). Immunoblotting for the serodiagnosis of alveolar echinococcosis in alive and dead Eurasian beavers (Castor fiber). Veterinary parasitology, 205(1-2), pp. 113-118. Elsevier 10.1016/j.vetpar.2014.06.017

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A novel species-specific anti-beaver-IgG-alkaline-phosphatase conjugate was synthesized for the development of a new serological test for echinococcosis in beavers. Two different ELISAs conventionally used for human Echinococcus multilocularis serology (Em18-ELISA and Em2-ELISA) yielded diagnostic sensitivities of 0% and 46%, respectively. In contrast, the subsequently developed immunoblotting assay gave an 85% diagnostic sensitivity (11 out of 13 beavers with alveolar echinococcosis were immunoblotting-positive, i.e. showed reactivity with a specific 21 Mr band), and maximal specificity. In conclusion, this immunoblotting assay should be the method of choice for use in serological studies on E. multilocularis in Eurasian beavers, and the test proved suitable to investigate both animals alive and post-mortem.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Center for Fish and Wildlife Health (FIWI)

UniBE Contributor:

Gottstein, Bruno; Frey, Caroline; Hentrich, Brigitte and Ryser, Marie Pierre

Subjects:

600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

0304-4017

Publisher:

Elsevier

Language:

English

Submitter:

Bruno Gottstein

Date Deposited:

16 Feb 2015 12:03

Last Modified:

22 Mar 2016 15:54

Publisher DOI:

10.1016/j.vetpar.2014.06.017

PubMed ID:

24986466

Uncontrolled Keywords:

Echinococcus multilocularis, Beaver, Em2-ELISA, Em18-ELISA, Immunoblotting, EmVF-antigen

BORIS DOI:

10.7892/boris.63067

URI:

https://boris.unibe.ch/id/eprint/63067

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