Endothelial binding of beta toxin to small intestinal mucosal endothelial cells in early stages of experimentally induced Clostridium perfringens type C enteritis in pigs.

Schumacher, Vanessa; Martel, A; Pasmans, F; Van Immerseel, F; Posthaus, Horst (2013). Endothelial binding of beta toxin to small intestinal mucosal endothelial cells in early stages of experimentally induced Clostridium perfringens type C enteritis in pigs. Veterinary pathology, 50(4), pp. 626-629. American College of Veterinary Pathologists 10.1177/0300985812461362

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Beta toxin (CPB) is known to be an essential virulence factor in the development of lesions of Clostridium perfringens type C enteritis in different animal species. Its target cells and exact mechanism of toxicity have not yet been clearly defined. Here, we evaluate the suitability of a neonatal piglet jejunal loop model to investigate early lesions of C. perfringens type C enteritis. Immunohistochemically, CPB was detected at microvascular endothelial cells in intestinal villi during early and advanced stages of lesions induced by C. perfringens type C. This was first associated with capillary dilatation and subsequently with widespread hemorrhage in affected intestinal segments. CPB was, however, not demonstrated on intestinal epithelial cells. This indicates a tropism of CPB toward endothelial cells and suggests that CPB-induced endothelial damage plays an important role in the early stages of C. perfringens type C enteritis in pigs.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Animal Pathology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Schumacher, Vanessa and Posthaus, Horst

Subjects:

600 Technology > 630 Agriculture

ISSN:

0300-9858

Publisher:

American College of Veterinary Pathologists

Language:

English

Submitter:

Susanne Portner

Date Deposited:

17 Feb 2015 16:16

Last Modified:

14 Oct 2015 11:33

Publisher DOI:

10.1177/0300985812461362

PubMed ID:

23012387

Uncontrolled Keywords:

Clostridium perfringens type C, beta toxin, endothelial cell, experimental infection, immunohistochemistry, intestinal loop model, necrotizing enteritis, porcine

BORIS DOI:

10.7892/boris.63151

URI:

https://boris.unibe.ch/id/eprint/63151

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