Covalent immobilisation of VEGF on plasma-coated electrospun scaffolds for tissue engineering applications.

Guex, A G; Hegemann, D; Giraud, Marie-Noelle; Tevaearai, Hendrik; Popa, A M; Rossi, R M; Fortunato, G (2014). Covalent immobilisation of VEGF on plasma-coated electrospun scaffolds for tissue engineering applications. Colloids and Surfaces B: Biointerfaces, 123, pp. 724-733. Elsevier 10.1016/j.colsurfb.2014.10.016

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Recent findings in the field of biomaterials and tissue engineering provide evidence that surface immobilised growth factors display enhanced stability and induce prolonged function. Cell response can be regulated by material properties and at the site of interest. To this end, we developed scaffolds with covalently bound vascular endothelial growth factor (VEGF) and evaluated their mitogenic effect on endothelial cells in vitro. Nano- (254±133 nm) or micro-fibrous (4.0±0.4 μm) poly(ɛ-caprolactone) (PCL) non-wovens were produced by electrospinning and coated in a radio frequency (RF) plasma process to induce an oxygen functional hydrocarbon layer. Implemented carboxylic acid groups were converted into amine-reactive esters and covalently coupled to VEGF by forming stable amide bonds (standard EDC/NHS chemistry). Substrates were analysed by X-ray photoelectron spectroscopy (XPS), enzyme-linked immuno-assays (ELISA) and immunohistochemistry (anti-VEGF antibody and VEGF-R2 binding). Depending on the reaction conditions, immobilised VEGF was present at 127±47 ng to 941±199 ng per substrate (6mm diameter; concentrations of 4.5 ng mm(-2) or 33.3 ng mm(-2), respectively). Immunohistochemistry provided evidence for biological integrity of immobilised VEGF. Endothelial cell number of primary endothelial cells or immortalised endothelial cells were significantly enhanced on VEGF-functionalised scaffolds compared to native PCL scaffolds. This indicates a sustained activity of immobilised VEGF over a culture period of nine days. We present a versatile method for the fabrication of growth factor-loaded scaffolds at specific concentrations.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Cardiovascular Disorders (DHGE) > Clinic of Cardiovascular Surgery

UniBE Contributor:

Giraud, Marie-Noelle and Tevaearai, Hendrik

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0927-7765

Publisher:

Elsevier

Language:

English

Submitter:

Sara Baumberger

Date Deposited:

17 Feb 2015 08:40

Last Modified:

27 Oct 2015 11:27

Publisher DOI:

10.1016/j.colsurfb.2014.10.016

PubMed ID:

25454657

Uncontrolled Keywords:

Electrospinning, Plasma polymerization, Tissue engineering, VEGF

BORIS DOI:

10.7892/boris.63188

URI:

https://boris.unibe.ch/id/eprint/63188

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