Optogenetics: opsins and optical interfaces in neuroscience.

Adamantidis, Antoine Roger; Zhang, Feng; de Lecea, Luis; Deisseroth, Karl (2014). Optogenetics: opsins and optical interfaces in neuroscience. Cold Spring Harbor protocols, 2014(8), pp. 815-822. Cold Spring Harbor Laboratory 10.1101/pdb.top083329

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Optogenetics is defined as the integration of optics and genetics to control well-defined events within specified cells of living tissue. In this introduction, we focus on the basic techniques necessary for employing microbial opsins as optogenetic tools in mammalian brains. We provide a guide for the fundamentals of optogenetic application-selecting an opsin, implementing expression of opsins based on the neuroscientific experimental requirements, and adapting the corresponding optical hardware for delivery of light into mammalian brains.

Item Type:

Journal Article (Review Article)

Division/Institute:

04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Neurology

UniBE Contributor:

Adamantidis, Antoine Roger

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1940-3402

Publisher:

Cold Spring Harbor Laboratory

Language:

English

Submitter:

Valentina Rossetti

Date Deposited:

27 Feb 2015 10:41

Last Modified:

27 Feb 2015 10:41

Publisher DOI:

10.1101/pdb.top083329

PubMed ID:

25086025

BORIS DOI:

10.7892/boris.63670

URI:

https://boris.unibe.ch/id/eprint/63670

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