The FgfrL1 receptor is required for development of slow muscle fibers.

Amann, Ruth; Wyder, Stefan; Slavotinek, Anne M; Trueb, Beat (2014). The FgfrL1 receptor is required for development of slow muscle fibers. Developmental biology, 394(2), pp. 228-241. Elsevier 10.1016/j.ydbio.2014.08.016

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FgfrL1, which interacts with Fgf ligands and heparin, is a member of the fibroblast growth factor receptor (Fgfr) family. FgfrL1-deficient mice show two significant alterations when compared to wildtype mice: They die at birth due to a malformed diaphragm and they lack metanephric kidneys. Utilizing gene arrays, qPCR and in situ hybridization we show here that the diaphragm of FgfrL1 knockout animals lacks any slow muscle fibers at E18.5 as indicated by the absence of slow fiber markers Myh7, Myl2 and Myl3. Similar lesions are also found in other skeletal muscles that contain a high proportion of slow fibers at birth, such as the extraocular muscles. In contrast to the slow fibers, fast fibers do not appear to be affected as shown by expression of fast fiber markers Myh3, Myh8, Myl1 and MylPF. At early developmental stages (E10.5, E15.5), FgfrL1-deficient animals express slow fiber genes at normal levels. The loss of slow fibers cannot be attributed to the lack of kidneys, since Wnt4 knockout mice, which also lack metanephric kidneys, show normal expression of Myh7, Myl2 and Myl3. Thus, FgfrL1 is specifically required for embryonic development of slow muscle fibers.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Rheumatology, Clinical Immunology and Allergology > Rheumatology (discontinued)
04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Rheumatology, Clinical Immunology and Allergology

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Amann, Ruth; Wyder, Stefan and Trueb, Beat

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0012-1606

Publisher:

Elsevier

Language:

English

Submitter:

Stefan Kuchen

Date Deposited:

06 Mar 2015 14:28

Last Modified:

06 Mar 2015 14:28

Publisher DOI:

10.1016/j.ydbio.2014.08.016

PubMed ID:

25172430

Uncontrolled Keywords:

Diaphragm, Extraocular muscles, Fgfr, FgfrL1, Fibroblast growth factor, Fibroblast growth factor receptor, Muscle development, Slow muscle fibers

BORIS DOI:

10.7892/boris.64168

URI:

https://boris.unibe.ch/id/eprint/64168

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