Uptake efficiency of surface modified gold nanoparticles does not correlate with functional changes and cytokine secretion in human dendritic cells in vitro.

Fytianos, Kleanthis; Rodriguez-Lorenzo, Laura; Clift, Martin J D; Blank, Fabian; Vanhecke, Dimitri; Von Garnier, Christophe; Petri-Fink, Alke; Rothen-Rutishauser, Barbara (2014). Uptake efficiency of surface modified gold nanoparticles does not correlate with functional changes and cytokine secretion in human dendritic cells in vitro. Nanomedicine : nanotechnology, biology, and medicine, 11(3), pp. 633-644. Elsevier 10.1016/j.nano.2014.11.004

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Engineering nanoparticles (NPs) for immune modulation require a thorough understanding of their interaction(s) with cells. Gold NPs (AuNPs) were coated with polyethylene glycol (PEG), polyvinyl alcohol (PVA) or a mixture of both with either positive or negative surface charge to investigate uptake and cell response in monocyte-derived dendritic cells (MDDCs). Inductively coupled plasma optical emission spectrometry and transmission electron microscopy were used to confirm the presence of Au inside MDDCs. Cell viability, (pro-)inflammatory responses, MDDC phenotype, activation markers, antigen uptake and processing were analyzed. Cell death was only observed for PVA-NH2 AuNPs at the highest concentration. MDDCs internalize AuNPs, however, surface modification influenced uptake. Though limited uptake was observed for PEG-COOH AuNPs, a significant tumor necrosis factor-alpha release was induced. In contrast, (PEG+PVA)-NH2 and PVA-NH2 AuNPs were internalized to a higher extent and caused interleukin-1beta secretion. None of the AuNPs caused changes in MDDC phenotype, activation or immunological properties.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Pneumologie (Erwachsene)
04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Pneumology

UniBE Contributor:

Blank, Fabian; Von Garnier, Christophe and Rothen-Rutishauser, Barbara

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1549-9642

Publisher:

Elsevier

Language:

English

Submitter:

Rahel Holderegger

Date Deposited:

10 Mar 2015 09:11

Last Modified:

29 Oct 2015 10:59

Publisher DOI:

10.1016/j.nano.2014.11.004

PubMed ID:

25555350

Uncontrolled Keywords:

Cell viability, Cellular uptake, Dendritic cells, Gold nanoparticles, Immunological properties

BORIS DOI:

10.7892/boris.64393

URI:

https://boris.unibe.ch/id/eprint/64393

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