Persistent and compartmentalised disruption of dendritic cell subpopulations in the lung following influenza A virus infection.

Strickland, Deborah H; Fear, Vanessa; Shenton, Seth; Wikstrom, Mathew E; Zosky, Graeme; Larcombe, Alexander N; Holt, Patrick G; Berry, Cassandra; Von Garnier, Christophe; Stumbles, Philip A (2014). Persistent and compartmentalised disruption of dendritic cell subpopulations in the lung following influenza A virus infection. PLoS ONE, 9(11), e111520. Public Library of Science 10.1371/journal.pone.0111520

[img]
Preview
Text
http___www.plosone.org_article_fetchObject.action_uri=info_doi_10.1371_journal.pone.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (1MB) | Preview

Immunological homeostasis in the respiratory tract is thought to require balanced interactions between networks of dendritic cell (DC) subsets in lung microenvironments in order to regulate tolerance or immunity to inhaled antigens and pathogens. Influenza A virus (IAV) poses a serious threat of long-term disruption to this balance through its potent pro-inflammatory activities. In this study, we have used a BALB/c mouse model of A/PR8/34 H1N1 Influenza Type A Virus infection to examine the effects of IAV on respiratory tissue DC subsets during the recovery phase following clearance of the virus. In adult mice, we found differences in the kinetics and activation states of DC residing in the airway mucosa (AMDC) compared to those in the parenchymal lung (PLDC) compartments. A significant depletion in the percentage of AMDC was observed at day 4 post-infection that was associated with a change in steady-state CD11b+ and CD11b- AMDC subset frequencies and significantly elevated CD40 and CD80 expression and that returned to baseline by day 14 post-infection. In contrast, percentages and total numbers of PLDC were significantly elevated at day 14 and remained so until day 21 post-infection. Accompanying this was a change in CD11b+and CD11b- PLDC subset frequencies and significant increase in CD40 and CD80 expression at these time points. Furthermore, mice infected with IAV at 4 weeks of age showed a significant increase in total numbers of PLDC, and increased CD40 expression on both AMDC and PLDC, when analysed as adults 35 days later. These data suggest that the rate of recovery of DC populations following IAV infection differs in the mucosal and parenchymal compartments of the lung and that DC populations can remain disrupted and activated for a prolonged period following viral clearance, into adulthood if infection occurred early in life.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Pneumologie (Erwachsene)
04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Pneumology

UniBE Contributor:

Von Garnier, Christophe

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1932-6203

Publisher:

Public Library of Science

Language:

English

Submitter:

Rahel Holderegger

Date Deposited:

10 Mar 2015 11:15

Last Modified:

20 Apr 2015 13:28

Publisher DOI:

10.1371/journal.pone.0111520

PubMed ID:

25398128

BORIS DOI:

10.7892/boris.64395

URI:

https://boris.unibe.ch/id/eprint/64395

Actions (login required)

Edit item Edit item
Provide Feedback