Enantioselective determination of ondansetron and 8-hydroxyondansetron in human plasma from recovered surgery patients by liquid chromatography-tandem mass spectrometry

Musshoff, F; Madea, B; Stüber, F; Stamer, U M (2010). Enantioselective determination of ondansetron and 8-hydroxyondansetron in human plasma from recovered surgery patients by liquid chromatography-tandem mass spectrometry. Journal of analytical toxicology, 34(9), pp. 581-6. Cary, N.C.: Oxford University Press 10.1093/jat/34.9.581

[img]
Preview
Text
34-9-581.pdf - Published Version
Available under License BORIS Standard License.

Download (368kB) | Preview

A liquid chromatographic-mass spectrometric assay with atmospheric pressure chemical ionization for quantification of ondansetron and its main metabolite 8-hydroxyondansetron in human plasma was presented. The enantiomeric separation was achieved on a Chiralcel OD-R column containing cellulose tris-(3,5-dimethylphenylcarbamate). The validation data were within the required limits. The assay was successfully applied to authentic plasma samples. Quantitative results from postoperative patients receiving ondansetron demonstrated a great interindividual variability in postoperative plasma drug concentrations, the metabolites were not detected in their unconjugated form. A wide variation in the S-(+)-/R-(-)-ondansetron concentration ratio between 0.14 and 7.18 is indicative for a stereoselective disposition or metabolism. In further studies CYP2D6 and CYP3A4 genotype dependent metabolism of ondansetron enantiomers as well as of co-administered drugs and clinical efficacy of the medication should be tested.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Intensive Care, Emergency Medicine and Anaesthesiology (DINA) > Clinic and Policlinic for Anaesthesiology and Pain Therapy

UniBE Contributor:

Stüber, Frank and Stamer, Ulrike

ISSN:

0146-4760

Publisher:

Oxford University Press

Language:

English

Submitter:

Jeannie Wurz

Date Deposited:

04 Oct 2013 14:08

Last Modified:

27 Apr 2018 11:23

Publisher DOI:

10.1093/jat/34.9.581

PubMed ID:

21073811

Web of Science ID:

000283891700008

BORIS DOI:

10.7892/boris.644

URI:

https://boris.unibe.ch/id/eprint/644 (FactScience: 200040)

Actions (login required)

Edit item Edit item
Provide Feedback