Quantification of nanoparticles at the single-cell level: an overview about state-of-the-art techniques and their limitations.

Vanhecke, Dimitri; Rodriguez-Lorenzo, Laura; Clift, Martin J D; Blank, Fabian; Petri-Fink, Alke; Rothen-Rutishauser, Barbara (2014). Quantification of nanoparticles at the single-cell level: an overview about state-of-the-art techniques and their limitations. Nanomedicine, 9(12), pp. 1885-1900. Future Medicine 10.2217/nnm.14.108

Full text not available from this repository. (Request a copy)

With the increasing production and use of engineered nanoparticles it is crucial that their interaction with biological systems is understood. Due to the small size of nanoparticles, their identification and localization within single cells is extremely challenging. Therefore, various cutting-edge techniques are required to detect and to quantify metals, metal oxides, magnetic, fluorescent, as well as electron-dense nanoparticles. Several techniques will be discussed in detail, such as inductively coupled plasma atomic emission spectroscopy, flow cytometry, laser scanning microscopy combined with digital image restoration, as well as quantitative analysis by means of stereology on transmission electron microscopy images. An overview will be given regarding the advantages of those visualization/quantification systems, including a thorough discussion about limitations and pitfalls.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Pneumologie (Erwachsene)
04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Pneumology

UniBE Contributor:

Blank, Fabian and Rothen-Rutishauser, Barbara

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1748-6963

Publisher:

Future Medicine

Language:

English

Submitter:

Rahel Holderegger

Date Deposited:

10 Mar 2015 11:10

Last Modified:

29 Oct 2015 11:00

Publisher DOI:

10.2217/nnm.14.108

PubMed ID:

25325243

Uncontrolled Keywords:

light microscopy, nanoparticles, quantification, single-cell level, spectroscopy, transmission electron microscopy

URI:

https://boris.unibe.ch/id/eprint/64403

Actions (login required)

Edit item Edit item
Provide Feedback