Can in-vitro chemoresponse assays help find new treatment regimens for malignant gliomas?

Linz, Ute; Ulus, Baran; Neuloh, Georg; Clusmann, Hans; Oertel, Markus Florian; Nolte, Kay; Weis, Joachim; Heussen, Nicole; Gilsbach, Joachim M (2014). Can in-vitro chemoresponse assays help find new treatment regimens for malignant gliomas? Anti-cancer drugs, 25(4), pp. 375-384. Lippincott Williams & Wilkins 10.1097/CAD.0000000000000062

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Various in-vitro chemosensitivity and resistance assays (CSRAs) have been demonstrated to be helpful decision aids for non-neurological tumors. Here, we evaluated the performance characteristics of two CSRAs for glioblastoma (GB) cells. The chemoresponse of fresh GB cells from 30 patients was studied in vitro using the ATP tumor chemoresponse assay and the chemotherapy resistance assay (CTR-Test). Both assay platforms provided comparable results. Of seven different chemotherapeutic drugs and drug combinations tested in vitro, treosulfan plus cytarabine (TARA) was the most effective, followed by nimustine (ACNU) plus teniposide (VM26) and temozolomide (TMZ). Whereas ACNU/VM26 and TMZ have proven their clinical value for malignant gliomas in large randomized studies, TARA has not been successful in newly diagnosed gliomas. This seeming discrepancy between in vitro and clinical result might be explained by the pharmacological behavior of treosulfan. Our results show reasonable agreement between two cell-based CSRAs. They appear to confirm the clinical effectiveness of drugs used in GB treatment as long as pharmacological preconditions such as overcoming the blood-brain barrier are properly considered.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Neurosurgery

UniBE Contributor:

Oertel, Markus Florian

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0959-4973

Publisher:

Lippincott Williams & Wilkins

Language:

English

Submitter:

Nicole Söll

Date Deposited:

13 Mar 2015 16:56

Last Modified:

01 May 2015 02:30

Publisher DOI:

10.1097/CAD.0000000000000062

PubMed ID:

24423983

BORIS DOI:

10.7892/boris.64534

URI:

https://boris.unibe.ch/id/eprint/64534

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