Fluorescence lifetime imaging of the ocular fundus in mice

Dysli, Chantal-Simone; Dysli, Denise-Muriel; Enzmann, Volker; Wolf, Sebastian; Zinkernagel, Martin (2014). Fluorescence lifetime imaging of the ocular fundus in mice. Investigative ophthalmology & visual science, 55(11), pp. 7206-7215. Association for Research in Vision and Ophthalmology 10.1167/iovs.14-14445

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Fundus autofluorescence (AF) is characterized not only by its intensity or excitation and emission spectra but also by the lifetimes of the fluorophores. Fluorescence lifetime is influenced by the fluorophore's microenvironment and may provide information about the metabolic tissue state. We report quantitative and qualitative autofluorescence lifetime imaging of the ocular fundus in mice.


A fluorescence lifetime imaging ophthalmoscope (FLIO) was used to measure fluorescence lifetimes of endogenous fluorophores in the murine retina. FLIO imaging was performed in 1-month-old C57BL/6, BALB/c, and C3A.Cg-Pde6b(+)Prph2(Rd2)/J mice. Measurements were repeated at monthly intervals over the course of 6 months. For correlation with structural changes, an optical coherence tomogram was acquired.


Fundus autofluorescence lifetime images were readily obtained in all mice. In the short spectral channel (498-560 nm), mean ± SEM AF lifetimes were 956 ± 15 picoseconds (ps) in C57BL/6; 801 ± 35 ps in BALB/c mice; and 882 ± 37 ps in C3A.Cg-Pde6b(+)Prph2(Rd2)/J mice. In the long spectral channel (560-720 nm), mean ± SEM AF lifetimes were 298 ± 14 ps in C57BL/6 mice, 241 ± 10 ps in BALB/c mice, and 288 ± 8 ps in C3A.Cg-Pde6b(+)Prph2(Rd2)/J mice. There was a general decrease in mean AF lifetimes with age.


Although fluorescence lifetime values differ among mouse strains, we found little variance within the groups. Fundus autofluorescence lifetime imaging in mice may provide additional information for understanding retinal disease processes and may facilitate monitoring of therapeutic effects in preclinical studies.

Item Type:

Journal Article (Original Article)


04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Augenklinik > Forschungsgruppe Augenheilkunde
04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Ophthalmology

UniBE Contributor:

Dysli, Chantal-Simone, Dysli, Denise-Muriel, Enzmann, Volker, Wolf, Sebastian (B), Zinkernagel, Martin Sebastian


600 Technology > 610 Medicine & health
500 Science




Association for Research in Vision and Ophthalmology




Sebastian Wolf

Date Deposited:

16 Mar 2015 12:12

Last Modified:

02 Mar 2023 23:26

Publisher DOI:


PubMed ID:


Uncontrolled Keywords:

fluorescence lifetimes, fundus autofluorescence, mouse models, ophthalmic imaging, validation studies





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