Fluorescence lifetime imaging of the ocular fundus in mice

Dysli, Chantal-Simone; Dysli, Denise-Muriel; Enzmann, Volker; Wolf, Sebastian; Zinkernagel, Martin (2014). Fluorescence lifetime imaging of the ocular fundus in mice. Investigative ophthalmology & visual science, 55(11), pp. 7206-7215. Association for Research in Vision and Ophthalmology 10.1167/iovs.14-14445

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PURPOSE Fundus autofluorescence (AF) is characterized not only by its intensity or excitation and emission spectra but also by the lifetimes of the fluorophores. Fluorescence lifetime is influenced by the fluorophore's microenvironment and may provide information about the metabolic tissue state. We report quantitative and qualitative autofluorescence lifetime imaging of the ocular fundus in mice. METHODS A fluorescence lifetime imaging ophthalmoscope (FLIO) was used to measure fluorescence lifetimes of endogenous fluorophores in the murine retina. FLIO imaging was performed in 1-month-old C57BL/6, BALB/c, and C3A.Cg-Pde6b(+)Prph2(Rd2)/J mice. Measurements were repeated at monthly intervals over the course of 6 months. For correlation with structural changes, an optical coherence tomogram was acquired. RESULTS Fundus autofluorescence lifetime images were readily obtained in all mice. In the short spectral channel (498-560 nm), mean ± SEM AF lifetimes were 956 ± 15 picoseconds (ps) in C57BL/6; 801 ± 35 ps in BALB/c mice; and 882 ± 37 ps in C3A.Cg-Pde6b(+)Prph2(Rd2)/J mice. In the long spectral channel (560-720 nm), mean ± SEM AF lifetimes were 298 ± 14 ps in C57BL/6 mice, 241 ± 10 ps in BALB/c mice, and 288 ± 8 ps in C3A.Cg-Pde6b(+)Prph2(Rd2)/J mice. There was a general decrease in mean AF lifetimes with age. CONCLUSIONS Although fluorescence lifetime values differ among mouse strains, we found little variance within the groups. Fundus autofluorescence lifetime imaging in mice may provide additional information for understanding retinal disease processes and may facilitate monitoring of therapeutic effects in preclinical studies.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Augenklinik > Forschungsgruppe Augenheilkunde
04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Ophthalmology

UniBE Contributor:

Dysli, Chantal-Simone; Dysli, Denise-Muriel; Enzmann, Volker; Wolf, Sebastian and Zinkernagel, Martin

Subjects:

600 Technology > 610 Medicine & health
500 Science

ISSN:

0146-0404

Publisher:

Association for Research in Vision and Ophthalmology

Language:

English

Submitter:

Sebastian Wolf

Date Deposited:

16 Mar 2015 12:12

Last Modified:

22 Dec 2015 13:11

Publisher DOI:

10.1167/iovs.14-14445

PubMed ID:

25249601

Uncontrolled Keywords:

fluorescence lifetimes, fundus autofluorescence, mouse models, ophthalmic imaging, validation studies

BORIS DOI:

10.7892/boris.64679

URI:

https://boris.unibe.ch/id/eprint/64679

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