Irisin – a myth rather than an exercise-inducible myokine

Albrecht, Elke; Norheim, Frode; Thiede, Bernd; Holen, Torgeir; Ohashi, Tomoo; Schering, Lisa; Lee, Sindre; Brenmoehl, Julia; Thomas, Selina; Drevon, Christian A.; Erickson, Harold P.; Maak, Steffen (2015). Irisin – a myth rather than an exercise-inducible myokine. Scientific Reports, 5, p. 8889. Nature Publishing Group 10.1038/srep08889

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The myokine irisin is supposed to be cleaved from a transmembrane precursor, FNDC5 (fibronectin type III domain containing 5), and to mediate beneficial effects of exercise on human metabolism. However, evidence for irisin circulating in blood is largely based on commercial ELISA kits which are based on polyclonal antibodies (pAbs) not previously tested for cross-reacting serum proteins. We have analyzed four commercial pAbs by Western blotting, which revealed prominent cross-reactivity with non-specific proteins in human and animal sera. Using recombinant glycosylated and non-glycosylated irisin as positive controls, we found no immune-reactive bands of the expected size in any biological samples. A FNDC5 signature was identified at ~20 kDa by mass spectrometry in human serum but was not detected by the commercial pAbs tested. Our results call into question all previous data obtained with commercial ELISA kits for irisin, and provide evidence against a physiological role for irisin in humans and other species.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Equine Clinic
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV)

UniBE Contributor:

Thomas, Selina

Subjects:

600 Technology > 630 Agriculture

ISSN:

2045-2322

Publisher:

Nature Publishing Group

Language:

English

Submitter:

Andrea Gassmann-Suter

Date Deposited:

19 Mar 2015 10:35

Last Modified:

10 Sep 2017 07:06

Publisher DOI:

10.1038/srep08889

PubMed ID:

25749243

BORIS DOI:

10.7892/boris.64866

URI:

https://boris.unibe.ch/id/eprint/64866

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