Reversibility of endocrine disruption in zebrafish (Danio rerio) after discontinued exposure to the estrogen 17α-ethinylestradiol.

Baumann, Lisa Annie; Knörr, Susanne; Keiter, Susanne; Rehberger, Kristina; Volz, Sina; Schiller, Viktoria; Fenske, Martina; Holbech, Henrik; Segner, Helmut; Braunbeck, Thomas (2014). Reversibility of endocrine disruption in zebrafish (Danio rerio) after discontinued exposure to the estrogen 17α-ethinylestradiol. Toxicology and Applied Pharmacology, 278(3), pp. 230-237. Elsevier 10.1016/j.taap.2014.04.025

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The aim of the present study was to investigate the persistence of the feminizing effects of discontinued 17α-ethinylestradiol (EE2) exposure on zebrafish (Danio rerio). An exposure scenario covering the sensitive phase of sexual differentiation, as well as final gonad maturation was chosen to examine the estrogenic effects on sexual development of zebrafish. Two exposure scenarios were compared: continuous exposure to environmentally relevant concentrations (0.1-10 ng/L EE2) up to 100 days post-hatch (dph) and developmental exposure up to 60 dph, followed by 40 days of depuration in clean water. The persistence of effects was investigated at different biological organization levels from mRNA to population-relevant endpoints to cover a broad range of important parameters. EE2 had a strong feminizing and inhibiting effect on the sexual development of zebrafish. Brain aromatase (cyp19b) mRNA expression showed no clear response, but vitellogenin levels were significantly elevated, gonad maturation and body growth were inhibited in both genders, and sex ratios were skewed towards females and undifferentiated individuals. To a large extent, all of these effects were reversed after 40 days of recovery, leading to the conclusion that exposure to the estrogen EE2 results in very strong, but reversible underdevelopment and feminization of zebrafish. The present study is the first to show this reversibility at different levels of organization, which gives better insight into the mechanistic basis of estrogenic effects in zebrafish.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Center for Fish and Wildlife Health (FIWI)

UniBE Contributor:

Baumann, Lisa Annie; Rehberger, Kristina and Segner, Helmut

Subjects:

600 Technology > 630 Agriculture

ISSN:

0041-008X

Publisher:

Elsevier

Language:

English

Submitter:

Lucia Gugger-Raaflaub

Date Deposited:

23 Apr 2015 10:55

Last Modified:

24 Nov 2016 17:08

Publisher DOI:

10.1016/j.taap.2014.04.025

PubMed ID:

24832493

Uncontrolled Keywords:

Aromatase, Endocrine disruptor, Estrogen, Gonad histology, Sexual differentiation, Vitellogenin

BORIS DOI:

10.7892/boris.65493

URI:

https://boris.unibe.ch/id/eprint/65493

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