SNARE-fusion mediated inserton of membrane proteins into native and artificial membranes

Nordlund, Gustav; Brzezinski, Peter; von Ballmoos, Christoph (July 2014). SNARE-fusion mediated inserton of membrane proteins into native and artificial membranes (Unpublished). In: European Bioenergetics Conference. Lisbon, Portugal. 12.-17.07.2014.

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Membrane proteins carry out functions such as nutrient uptake, ATP synthesis or transmembrane signal transduction. An increasing number of reports indicate that cellular processes are underpinned by regulated interactions between these proteins. Consequently, functional studies of these networks at a molecular level require co-reconstitution of the interacting components. Here, we report a SNARE-protein based method for incorporation of multiple membrane proteins into membranes, and for delivery of large water-soluble substrates into closed membrane vesicles. The approach is used for in vitro reconstruction of a fully functional bacterial respiratory chain from purified components. Furthermore, the method is used for functional incorporation of the entire F1F0-ATP synthase complex into native bacterial membranes from which this component had been genetically removed. The novel methodology offers a tool to investigate complex interaction networks between membrane-bound proteins at a molecular level, which is expected to generate functional insights into key cellular functions.

Item Type:

Conference or Workshop Item (Poster)

Division/Institute:

08 Faculty of Science > Departement of Chemistry and Biochemistry

UniBE Contributor:

von Ballmoos, Christoph

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry

Language:

English

Submitter:

Christoph von Ballmoos

Date Deposited:

08 Apr 2015 10:05

Last Modified:

08 Apr 2015 10:05

BORIS DOI:

10.7892/boris.65912

URI:

https://boris.unibe.ch/id/eprint/65912

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