An in situ surface electrochemistry approach towards whole-cell studies: the structure and reactivity of a Geobacter sulfurreducens submonolayer on electrified metal/electrolyte interfaces

Kuzume, Akiyoshi; Zhumaev, Ulmas; Li, Jianfeng; Fu, Yongchun; Füeg, Michael; Estévez, Marta; Borjas, Zulema; Wandlowski, Thomas; Esteve-Nuñez, Abraham (2014). An in situ surface electrochemistry approach towards whole-cell studies: the structure and reactivity of a Geobacter sulfurreducens submonolayer on electrified metal/electrolyte interfaces. Physical Chemistry Chemical Physics, 16(40), pp. 22229-22236. Royal Society of Chemistry 10.1039/C4CP03357D

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A direct electron transfer process between bacterial cells of electrogenic species Geobacter sulfurreducens (Gs) and electrified electrode surfaces was studied to exploit the reactivity of Gs submonolayers on gold and silver surfaces. A submonolayer of Gs was prepared and studied to explore specifically the heterogeneous electron transfer properties at the bacteria/electrode interface. In situ microscopic techniques characterised the morphology of the Gs submonolayers under the operating conditions. In addition, complementary in situ spectroscopic techniques that allowed us to access in situ molecular information of the Gs with high surface selectivity and sensitivity were employed. The results provided clear evidence that the outermost cytochrome C in Gs is responsible for the heterogeneous electron transfer, which is in direct contact with the metal electrode. Feasibility of single cell in situ studies under operating conditions was demonstrated where the combination of surface-electrochemical tools at the nano- and micro-scale with microbiological approaches can offer unique opportunities for the emerging field of electro-microbiology to explore processes and interactions between microorganisms and electrical devices.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Departement of Chemistry and Biochemistry

UniBE Contributor:

Kuzume, Akiyoshi; Zhumaev, Ulmas; Li, Jianfeng; Fu, Yongchun; Füeg, Michael and Wandlowski, Thomas

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry
500 Science

ISSN:

1463-9076

Publisher:

Royal Society of Chemistry

Language:

English

Submitter:

Beatrice Niederhauser

Date Deposited:

26 Mar 2015 10:15

Last Modified:

14 Jan 2016 09:06

Publisher DOI:

10.1039/C4CP03357D

BORIS DOI:

10.7892/boris.65929

URI:

https://boris.unibe.ch/id/eprint/65929

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